A charge-switched nano-sized polymeric carrier for protein delivery

Bo Reum Lee; Kyung Taek Oh; Hye Jung Baik; Yu Seok Youn; Eun Seong Lee

doi:10.1016/j.ijpharm.2010.03.028

A charge-switched nano-sized polymeric carrier for protein delivery

Bo Reum Lee, Kyung Taek Oh, Hye Jung Baik, Yu Seok Youn, Eun Seong Lee

Department of Biomedical-Chemical Engineering

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A novel synthetic nanocomplex was constructed from glycol chitosan (GCS) grafted with 2,3-dimethylmaleic anhydride (DMA) (denoted as 'GCD' hereafter) and lysozyme (isoelectric point=10.9) as a model protein. This is a core-shell supramolecular assemble formed through electrostatic interactions between anionic GCD and cationic lysozyme at a pH 7.4. The pH-sensitivity of the nanocomplexes originates from the dissociation of DMA block from GCD at a slightly acidic pH (i.e., pH 6.8), resulting in an increased electrostatic repulsion between cationic GCS and cationic lysozyme. This pH-induced charge switching of GCD provides a mechanism for triggered protein drug release from the nanocomplexes triggered by the small change in pH (pH 7.4-6.8).

Original language	English
Pages (from-to)	78-82
Number of pages	5
Journal	International Journal of Pharmaceutics
Volume	392
Issue number	1-2
DOIs	https://doi.org/10.1016/j.ijpharm.2010.03.028
State	Published - Jun 2010

Bibliographical note

Funding Information:
This work was supported by the Research Fund, 2009 of the Catholic University of Korea, by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( MEST ) (no. 2009-0083962 ), and by a grant from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea.

Keywords

Acidosis
Charge-switched nanocomplex
Protein delivery

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10.1016/j.ijpharm.2010.03.028

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title = "A charge-switched nano-sized polymeric carrier for protein delivery",

abstract = "A novel synthetic nanocomplex was constructed from glycol chitosan (GCS) grafted with 2,3-dimethylmaleic anhydride (DMA) (denoted as 'GCD' hereafter) and lysozyme (isoelectric point=10.9) as a model protein. This is a core-shell supramolecular assemble formed through electrostatic interactions between anionic GCD and cationic lysozyme at a pH 7.4. The pH-sensitivity of the nanocomplexes originates from the dissociation of DMA block from GCD at a slightly acidic pH (i.e., pH 6.8), resulting in an increased electrostatic repulsion between cationic GCS and cationic lysozyme. This pH-induced charge switching of GCD provides a mechanism for triggered protein drug release from the nanocomplexes triggered by the small change in pH (pH 7.4-6.8).",

keywords = "Acidosis, Charge-switched nanocomplex, Protein delivery",

author = "Lee, {Bo Reum} and Oh, {Kyung Taek} and Baik, {Hye Jung} and Youn, {Yu Seok} and Lee, {Eun Seong}",

note = "Funding Information: This work was supported by the Research Fund, 2009 of the Catholic University of Korea, by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( MEST ) (no. 2009-0083962 ), and by a grant from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea. ",

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doi = "10.1016/j.ijpharm.2010.03.028",

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AU - Oh, Kyung Taek

AU - Baik, Hye Jung

AU - Youn, Yu Seok

AU - Lee, Eun Seong

N1 - Funding Information: This work was supported by the Research Fund, 2009 of the Catholic University of Korea, by the National Research Foundation of Korea (NRF) grant funded by the Korea government ( MEST ) (no. 2009-0083962 ), and by a grant from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea.

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N2 - A novel synthetic nanocomplex was constructed from glycol chitosan (GCS) grafted with 2,3-dimethylmaleic anhydride (DMA) (denoted as 'GCD' hereafter) and lysozyme (isoelectric point=10.9) as a model protein. This is a core-shell supramolecular assemble formed through electrostatic interactions between anionic GCD and cationic lysozyme at a pH 7.4. The pH-sensitivity of the nanocomplexes originates from the dissociation of DMA block from GCD at a slightly acidic pH (i.e., pH 6.8), resulting in an increased electrostatic repulsion between cationic GCS and cationic lysozyme. This pH-induced charge switching of GCD provides a mechanism for triggered protein drug release from the nanocomplexes triggered by the small change in pH (pH 7.4-6.8).

AB - A novel synthetic nanocomplex was constructed from glycol chitosan (GCS) grafted with 2,3-dimethylmaleic anhydride (DMA) (denoted as 'GCD' hereafter) and lysozyme (isoelectric point=10.9) as a model protein. This is a core-shell supramolecular assemble formed through electrostatic interactions between anionic GCD and cationic lysozyme at a pH 7.4. The pH-sensitivity of the nanocomplexes originates from the dissociation of DMA block from GCD at a slightly acidic pH (i.e., pH 6.8), resulting in an increased electrostatic repulsion between cationic GCS and cationic lysozyme. This pH-induced charge switching of GCD provides a mechanism for triggered protein drug release from the nanocomplexes triggered by the small change in pH (pH 7.4-6.8).

KW - Acidosis

KW - Charge-switched nanocomplex

KW - Protein delivery

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DO - 10.1016/j.ijpharm.2010.03.028

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SN - 0378-5173

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EP - 82

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

IS - 1-2

ER -

A charge-switched nano-sized polymeric carrier for protein delivery

Abstract

Bibliographical note

Keywords

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