A comparative analysis of in vitro toxicity of synthetic zeolites on imr-90 human lung fibroblast cells

Seung Hye Yu; Manjesh Kumar; Il Won Kim; Jeffrey D. Rimer; Tae Jung Kim

doi:10.3390/molecules26113194

A comparative analysis of in vitro toxicity of synthetic zeolites on imr-90 human lung fibroblast cells

Seung Hye Yu, Manjesh Kumar, Il Won Kim, Jeffrey D. Rimer, Tae Jung Kim

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Broad industrial application of zeolites increases the opportunity of inhalation. However, the potential impact of different type and composition of zeolite on the cytotoxicity is still unknown. Four types of synthetic zeolites with have been prepared for assessing the effect on lung fibroblast: two zeolite L (LTL-R and LTL-D, ZSM-5 (MFI-S), and faujasite (FAU-S). The cytotoxicity of zeolites on human lung fibroblast (IMR-90) was assessed using WST1 cell proliferation assay, mitochondrial function, membrane leakage of lactate dehydrogenase, reduced glutathione levels, and mitochondrial membrane potential were assessed under control. Intracellular changes were examined using transmission electron microscopy (TEM). Toxicity related gene expression were evaluated by PCR array. The result showed a significantly higher toxicity in IMR-90 cells with FAU-S than LTL-R, LTL-D and MFI-S exposure. TEM showed FAU-S, spheroidal zeolite with a low Si/Al ratio, was readily internalized forming numerous phagosomes in IMR-90 cells, while the largest and disc-shaped zeolites showed the lowest toxicity and were located in submembranous phagosomes in IMR-90 cells. Differential expression of TNF related genes was detected using PCR arrays and confirmed using qRT-PCR analysis of selected genes. Collectively, the exposure of different zeolites shows different toxicity on IMR-90 cells.

Original language	English
Article number	3194
Journal	Molecules
Volume	26
Issue number	11
DOIs	https://doi.org/10.3390/molecules26113194
State	Published - 1 Jun 2021

Bibliographical note

Funding Information:
Acknowledgments: This study was supported by Institute of Clinical Medicine Research at Yeouido St. Mary’s Hospital.

Funding Information:
Funding: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1E1A1A01078335), and the Radiation Safety Materials and Medical Technology R&D program through the National Research Foundation of Korea funded by the Ministry of Science and ICT (NRF-2019M2C8A2058418). JDR acknowledges financial support from the Welch Foundation (Award E-1794).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Cytotoxicity
Fibroblast
Glutathione
IMR-90
Lung
Zeolite

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10.3390/molecules26113194

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title = "A comparative analysis of in vitro toxicity of synthetic zeolites on imr-90 human lung fibroblast cells",

abstract = "Broad industrial application of zeolites increases the opportunity of inhalation. However, the potential impact of different type and composition of zeolite on the cytotoxicity is still unknown. Four types of synthetic zeolites with have been prepared for assessing the effect on lung fibroblast: two zeolite L (LTL-R and LTL-D, ZSM-5 (MFI-S), and faujasite (FAU-S). The cytotoxicity of zeolites on human lung fibroblast (IMR-90) was assessed using WST1 cell proliferation assay, mitochondrial function, membrane leakage of lactate dehydrogenase, reduced glutathione levels, and mitochondrial membrane potential were assessed under control. Intracellular changes were examined using transmission electron microscopy (TEM). Toxicity related gene expression were evaluated by PCR array. The result showed a significantly higher toxicity in IMR-90 cells with FAU-S than LTL-R, LTL-D and MFI-S exposure. TEM showed FAU-S, spheroidal zeolite with a low Si/Al ratio, was readily internalized forming numerous phagosomes in IMR-90 cells, while the largest and disc-shaped zeolites showed the lowest toxicity and were located in submembranous phagosomes in IMR-90 cells. Differential expression of TNF related genes was detected using PCR arrays and confirmed using qRT-PCR analysis of selected genes. Collectively, the exposure of different zeolites shows different toxicity on IMR-90 cells.",

keywords = "Cytotoxicity, Fibroblast, Glutathione, IMR-90, Lung, Zeolite",

author = "Yu, {Seung Hye} and Manjesh Kumar and Kim, {Il Won} and Rimer, {Jeffrey D.} and Kim, {Tae Jung}",

note = "Funding Information: Acknowledgments: This study was supported by Institute of Clinical Medicine Research at Yeouido St. Mary{\textquoteright}s Hospital. Funding Information: Funding: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1E1A1A01078335), and the Radiation Safety Materials and Medical Technology R&D program through the National Research Foundation of Korea funded by the Ministry of Science and ICT (NRF-2019M2C8A2058418). JDR acknowledges financial support from the Welch Foundation (Award E-1794). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/molecules26113194",

language = "English",

volume = "26",

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AU - Kumar, Manjesh

AU - Kim, Il Won

AU - Rimer, Jeffrey D.

AU - Kim, Tae Jung

N1 - Funding Information: Acknowledgments: This study was supported by Institute of Clinical Medicine Research at Yeouido St. Mary’s Hospital. Funding Information: Funding: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1E1A1A01078335), and the Radiation Safety Materials and Medical Technology R&D program through the National Research Foundation of Korea funded by the Ministry of Science and ICT (NRF-2019M2C8A2058418). JDR acknowledges financial support from the Welch Foundation (Award E-1794). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/6/1

Y1 - 2021/6/1

N2 - Broad industrial application of zeolites increases the opportunity of inhalation. However, the potential impact of different type and composition of zeolite on the cytotoxicity is still unknown. Four types of synthetic zeolites with have been prepared for assessing the effect on lung fibroblast: two zeolite L (LTL-R and LTL-D, ZSM-5 (MFI-S), and faujasite (FAU-S). The cytotoxicity of zeolites on human lung fibroblast (IMR-90) was assessed using WST1 cell proliferation assay, mitochondrial function, membrane leakage of lactate dehydrogenase, reduced glutathione levels, and mitochondrial membrane potential were assessed under control. Intracellular changes were examined using transmission electron microscopy (TEM). Toxicity related gene expression were evaluated by PCR array. The result showed a significantly higher toxicity in IMR-90 cells with FAU-S than LTL-R, LTL-D and MFI-S exposure. TEM showed FAU-S, spheroidal zeolite with a low Si/Al ratio, was readily internalized forming numerous phagosomes in IMR-90 cells, while the largest and disc-shaped zeolites showed the lowest toxicity and were located in submembranous phagosomes in IMR-90 cells. Differential expression of TNF related genes was detected using PCR arrays and confirmed using qRT-PCR analysis of selected genes. Collectively, the exposure of different zeolites shows different toxicity on IMR-90 cells.

AB - Broad industrial application of zeolites increases the opportunity of inhalation. However, the potential impact of different type and composition of zeolite on the cytotoxicity is still unknown. Four types of synthetic zeolites with have been prepared for assessing the effect on lung fibroblast: two zeolite L (LTL-R and LTL-D, ZSM-5 (MFI-S), and faujasite (FAU-S). The cytotoxicity of zeolites on human lung fibroblast (IMR-90) was assessed using WST1 cell proliferation assay, mitochondrial function, membrane leakage of lactate dehydrogenase, reduced glutathione levels, and mitochondrial membrane potential were assessed under control. Intracellular changes were examined using transmission electron microscopy (TEM). Toxicity related gene expression were evaluated by PCR array. The result showed a significantly higher toxicity in IMR-90 cells with FAU-S than LTL-R, LTL-D and MFI-S exposure. TEM showed FAU-S, spheroidal zeolite with a low Si/Al ratio, was readily internalized forming numerous phagosomes in IMR-90 cells, while the largest and disc-shaped zeolites showed the lowest toxicity and were located in submembranous phagosomes in IMR-90 cells. Differential expression of TNF related genes was detected using PCR arrays and confirmed using qRT-PCR analysis of selected genes. Collectively, the exposure of different zeolites shows different toxicity on IMR-90 cells.

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KW - Fibroblast

KW - Glutathione

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KW - Lung

KW - Zeolite

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DO - 10.3390/molecules26113194

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SN - 1420-3049

VL - 26

JO - Molecules

JF - Molecules

IS - 11

M1 - 3194

ER -

A comparative analysis of in vitro toxicity of synthetic zeolites on imr-90 human lung fibroblast cells

Abstract

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Keywords

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