A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

Joon Ho Kim; Byoung Gyun Kim; Hyukjun Nam; Dae Eun Park; Kwang Seok Yun; Jun Bo Yoon; Jichang You; Euisik Yoon

doi:10.1109/MEMSYS.2002.984222

A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

Joon Ho Kim
, Byoung Gyun Kim
, Hyukjun Nam
, Dae Eun Park
, Kwang Seok Yun
, Jun Bo Yoon
, Jichang You
, Euisik Yoon

Department of Pathology

Korea Advanced Institute of Science and Technology
The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm² and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.

Original language	English
Pages (from-to)	133-136
Number of pages	4
Journal	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
DOIs	https://doi.org/10.1109/MEMSYS.2002.984222
State	Published - 2002

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title = "A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay",

abstract = "A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.",

author = "Kim, \{Joon Ho\} and Kim, \{Byoung Gyun\} and Hyukjun Nam and Park, \{Dae Eun\} and Yun, \{Kwang Seok\} and Yoon, \{Jun Bo\} and Jichang You and Euisik Yoon",

year = "2002",

doi = "10.1109/MEMSYS.2002.984222",

language = "English",

pages = "133--136",

journal = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

issn = "1084-6999",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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T1 - A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

AU - Kim, Joon Ho

AU - Kim, Byoung Gyun

AU - Nam, Hyukjun

AU - Park, Dae Eun

AU - Yun, Kwang Seok

AU - Yoon, Jun Bo

AU - You, Jichang

AU - Yoon, Euisik

PY - 2002

Y1 - 2002

N2 - A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.

AB - A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2μm diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynold numbers below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15mg/cm2 and a minimum extractable input concentration of 100 copies/200μL. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.

UR - https://www.scopus.com/pages/publications/0036198841

U2 - 10.1109/MEMSYS.2002.984222

DO - 10.1109/MEMSYS.2002.984222

M3 - Article

AN - SCOPUS:0036198841

SN - 1084-6999

SP - 133

EP - 136

JO - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

JF - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

ER -

A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

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