A naturally derived gastric cancer cell line shows latency I Epstein-Barr virus infection closely resembling EBV-associated gastric cancer

Sang Taek Oh; Jung Seon Seo; Uk Yeol Moon; Kyeong Hee Kang; Dong Jik Shin; Sungjoo Kim Yoon; Woo Ho Kim; Jae Gahb Park; Suk Kyeong Lee

doi:10.1016/j.virol.2003.12.005

A naturally derived gastric cancer cell line shows latency I Epstein-Barr virus infection closely resembling EBV-associated gastric cancer

Sang Taek Oh
, Jung Seon Seo
, Uk Yeol Moon
, Kyeong Hee Kang
, Dong Jik Shin
, Sungjoo Kim Yoon
, Woo Ho Kim
, Jae Gahb Park
, Suk Kyeong Lee

Department of Biomedicine & Health Science

Catholic University of Korea
Seoul National University

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

In a process seeking out a good model cell line for Epstein-Barr virus (EBV)-associated gastric cancer, we found that one previously established gastric adenocarcinoma cell line is infected with type 1 EBV. This SNU-719 cell line from a Korean patient expressed cytokeratin without CD19 or CD21 expression. In SNU-719, EBNA1 and LMP2A were expressed, while LMP1 and EBNA2 were not. None of the tested lytic EBV proteins were detected in this cell line unless stimulated with phorbol ester. EBV infection was also shown in the original carcinoma tissue of SNU-719 cell line. Our results support the possibility of a CD21-independent EBV infection of gastric epithelial cells in vivo. As the latent EBV gene expression pattern of SNU-719 closely resembles that of the EBV-associated gastric cancer, this naturally derived cell line may serve as a valuable model system to clarify the precise role of EBV in gastric carcinogenesis.

Original language	English
Pages (from-to)	330-336
Number of pages	7
Journal	Virology
Volume	320
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2003.12.005
State	Published - 15 Mar 2004

Bibliographical note

Funding Information:
We thank Drs. Hyo-Jeong Kuh, Suk-Chul Bae, and Jae-Yong Lee for providing cell lines at the initial stage of this study. We also thank Dr. Won-Keun Lee for providing a probe containing the terminal repeat sequence of EBV and anti-EBNA1 human serum. We are grateful to Sun Hee Suh for her valuable help in immunofluorescence assay. This work was supported by grant no. (R01-1999-000-00066-0) from the Basic Research Program of the Korea Science and Engineering Foundation and grants from the Catholic Cancer Center, Catholic University, Seoul, Korea.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell Line
EBV latency
Epstein-Barr virus
Gastric cancer

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10.1016/j.virol.2003.12.005

Cite this

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title = "A naturally derived gastric cancer cell line shows latency I Epstein-Barr virus infection closely resembling EBV-associated gastric cancer",

abstract = "In a process seeking out a good model cell line for Epstein-Barr virus (EBV)-associated gastric cancer, we found that one previously established gastric adenocarcinoma cell line is infected with type 1 EBV. This SNU-719 cell line from a Korean patient expressed cytokeratin without CD19 or CD21 expression. In SNU-719, EBNA1 and LMP2A were expressed, while LMP1 and EBNA2 were not. None of the tested lytic EBV proteins were detected in this cell line unless stimulated with phorbol ester. EBV infection was also shown in the original carcinoma tissue of SNU-719 cell line. Our results support the possibility of a CD21-independent EBV infection of gastric epithelial cells in vivo. As the latent EBV gene expression pattern of SNU-719 closely resembles that of the EBV-associated gastric cancer, this naturally derived cell line may serve as a valuable model system to clarify the precise role of EBV in gastric carcinogenesis.",

keywords = "Cell Line, EBV latency, Epstein-Barr virus, Gastric cancer",

author = "Oh, \{Sang Taek\} and Seo, \{Jung Seon\} and Moon, \{Uk Yeol\} and Kang, \{Kyeong Hee\} and Shin, \{Dong Jik\} and Yoon, \{Sungjoo Kim\} and Kim, \{Woo Ho\} and Park, \{Jae Gahb\} and Lee, \{Suk Kyeong\}",

note = "Funding Information: We thank Drs. Hyo-Jeong Kuh, Suk-Chul Bae, and Jae-Yong Lee for providing cell lines at the initial stage of this study. We also thank Dr. Won-Keun Lee for providing a probe containing the terminal repeat sequence of EBV and anti-EBNA1 human serum. We are grateful to Sun Hee Suh for her valuable help in immunofluorescence assay. This work was supported by grant no. (R01-1999-000-00066-0) from the Basic Research Program of the Korea Science and Engineering Foundation and grants from the Catholic Cancer Center, Catholic University, Seoul, Korea.",

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doi = "10.1016/j.virol.2003.12.005",

language = "English",

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journal = "Virology",

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T1 - A naturally derived gastric cancer cell line shows latency I Epstein-Barr virus infection closely resembling EBV-associated gastric cancer

AU - Oh, Sang Taek

AU - Seo, Jung Seon

AU - Moon, Uk Yeol

AU - Kang, Kyeong Hee

AU - Shin, Dong Jik

AU - Yoon, Sungjoo Kim

AU - Kim, Woo Ho

AU - Park, Jae Gahb

AU - Lee, Suk Kyeong

N1 - Funding Information: We thank Drs. Hyo-Jeong Kuh, Suk-Chul Bae, and Jae-Yong Lee for providing cell lines at the initial stage of this study. We also thank Dr. Won-Keun Lee for providing a probe containing the terminal repeat sequence of EBV and anti-EBNA1 human serum. We are grateful to Sun Hee Suh for her valuable help in immunofluorescence assay. This work was supported by grant no. (R01-1999-000-00066-0) from the Basic Research Program of the Korea Science and Engineering Foundation and grants from the Catholic Cancer Center, Catholic University, Seoul, Korea.

PY - 2004/3/15

Y1 - 2004/3/15

N2 - In a process seeking out a good model cell line for Epstein-Barr virus (EBV)-associated gastric cancer, we found that one previously established gastric adenocarcinoma cell line is infected with type 1 EBV. This SNU-719 cell line from a Korean patient expressed cytokeratin without CD19 or CD21 expression. In SNU-719, EBNA1 and LMP2A were expressed, while LMP1 and EBNA2 were not. None of the tested lytic EBV proteins were detected in this cell line unless stimulated with phorbol ester. EBV infection was also shown in the original carcinoma tissue of SNU-719 cell line. Our results support the possibility of a CD21-independent EBV infection of gastric epithelial cells in vivo. As the latent EBV gene expression pattern of SNU-719 closely resembles that of the EBV-associated gastric cancer, this naturally derived cell line may serve as a valuable model system to clarify the precise role of EBV in gastric carcinogenesis.

AB - In a process seeking out a good model cell line for Epstein-Barr virus (EBV)-associated gastric cancer, we found that one previously established gastric adenocarcinoma cell line is infected with type 1 EBV. This SNU-719 cell line from a Korean patient expressed cytokeratin without CD19 or CD21 expression. In SNU-719, EBNA1 and LMP2A were expressed, while LMP1 and EBNA2 were not. None of the tested lytic EBV proteins were detected in this cell line unless stimulated with phorbol ester. EBV infection was also shown in the original carcinoma tissue of SNU-719 cell line. Our results support the possibility of a CD21-independent EBV infection of gastric epithelial cells in vivo. As the latent EBV gene expression pattern of SNU-719 closely resembles that of the EBV-associated gastric cancer, this naturally derived cell line may serve as a valuable model system to clarify the precise role of EBV in gastric carcinogenesis.

KW - Cell Line

KW - EBV latency

KW - Epstein-Barr virus

KW - Gastric cancer

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DO - 10.1016/j.virol.2003.12.005

M3 - Article

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AN - SCOPUS:1542315566

SN - 0042-6822

VL - 320

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JF - Virology

IS - 2

ER -

A naturally derived gastric cancer cell line shows latency I Epstein-Barr virus infection closely resembling EBV-associated gastric cancer

Abstract

Bibliographical note

UN SDGs

Keywords

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