Abstract
Differentiation of tuberculous granuloma (TG) from non-tuberculous granuloma (NG) is histopathologically difficult. We evaluated the usefulness of selected immunohistochemical markers to differentiate tuberculous granuloma (TG) and non-tuberculous granuloma (NG). We selected six biomarkers (FoxP3, TNF-beta, E-selectin [ESEL], indoleamine 2,3-dioxygenase [IDO], lactoferrin [LACT], and tartrate-resistant acid phosphatase [TRAP]) and immunohistochemically analyzed their expression in the presence of two types of granulomatous tissue samples, TG (n = 36) and NG (n = 31), using a microarray format. Three of those six biomarkers (LACT, IDO, and TNF-beta) were moderately accurate in discriminating TG from NG, individually and in combination, according to ROC analysis (AUC = 0.7-0.89, sensitivity = 55.6-77.8%, specificity = 71.0-100%). Our data indicate that selected immunohistochemical markers (LACT, IDO, and TNF-beta) can be used in ancillary tests to differentiate TG from NG in tissue samples. Further large-scale studies are required to validate our results.
| Original language | English |
|---|---|
| Pages (from-to) | 13900-13910 |
| Number of pages | 11 |
| Journal | International Journal of Clinical and Experimental Pathology |
| Volume | 8 |
| Issue number | 11 |
| State | Published - 2015 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Biomarkers
- E-selectin (ESEL)
- Extrapulmonary tuberculosis (EPTB)
- FoxP3
- Granuloma
- Immunohistochemistry
- Indoleamine 2,3-dioxygenase (IDO)
- Lactoferrin (LACT)
- TNF-beta
- Tuberculosis
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