Activation of human natural killer cells by the soluble form of cellular prion protein

Yeon Jae Seong; Pil Soo Sung; Young Soon Jang; Young Joon Choi; Bum Chan Park; Su Hyung Park; Young Woo Park; Eui Cheol Shin

doi:10.1016/j.bbrc.2015.06.172

Activation of human natural killer cells by the soluble form of cellular prion protein

Yeon Jae Seong
, Pil Soo Sung
, Young Soon Jang
, Young Joon Choi
, Bum Chan Park
, Su Hyung Park
, Young Woo Park
, Eui Cheol Shin

Department of Internal Medicine

Korea Advanced Institute of Science and Technology
Hafis Clinic
Korea Research Institute of Bioscience and Biotechnology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Cellular prion protein (PrP^C) is widely expressed in various cell types, including cells of the immune system. However, the specific roles of PrP^C in the immune system have not been clearly elucidated. In the present study, we investigated the effects of a soluble form of recombinant PrP^C protein on human natural killer (NK) cells. Recombinant soluble PrP^C protein was generated by fusion of human PrP^C with the Fc portion of human IgG₁ (PrP^C-Fc). PrP^C-Fc binds to the surface of human NK cells, particularly to CD56^dim NK cells. PrP^C-Fc induced the production of cytokines and chemokines and the degranulation of granzyme B from NK cells. In addition, PrP^C-Fc facilitated the IL-15-induced proliferation of NK cells. PrP^C-Fc induced phosphorylation of ERK-1/2 and JNK in NK cells, and inhibitors of the ERK or the JNK pathways abrogated PrP^C-Fc-induced cytokine production in NK cells. In conclusion, the soluble form of recombinant PrP^C-Fc protein activates human NK cells via the ERK and JNK signaling pathways.

Original language	English
Pages (from-to)	512-518
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	464
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2015.06.172
State	Published - 30 Jul 2015

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Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

Keywords

Activation
Cellular prion protein
Cytokines
Natural killer cells

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10.1016/j.bbrc.2015.06.172

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@article{e42612578740453aad68d2be0133755c,

title = "Activation of human natural killer cells by the soluble form of cellular prion protein",

abstract = "Cellular prion protein (PrPC) is widely expressed in various cell types, including cells of the immune system. However, the specific roles of PrPC in the immune system have not been clearly elucidated. In the present study, we investigated the effects of a soluble form of recombinant PrPC protein on human natural killer (NK) cells. Recombinant soluble PrPC protein was generated by fusion of human PrPC with the Fc portion of human IgG1 (PrPC-Fc). PrPC-Fc binds to the surface of human NK cells, particularly to CD56dim NK cells. PrPC-Fc induced the production of cytokines and chemokines and the degranulation of granzyme B from NK cells. In addition, PrPC-Fc facilitated the IL-15-induced proliferation of NK cells. PrPC-Fc induced phosphorylation of ERK-1/2 and JNK in NK cells, and inhibitors of the ERK or the JNK pathways abrogated PrPC-Fc-induced cytokine production in NK cells. In conclusion, the soluble form of recombinant PrPC-Fc protein activates human NK cells via the ERK and JNK signaling pathways.",

keywords = "Activation, Cellular prion protein, Cytokines, Natural killer cells",

author = "Seong, \{Yeon Jae\} and Sung, \{Pil Soo\} and Jang, \{Young Soon\} and Choi, \{Young Joon\} and Park, \{Bum Chan\} and Park, \{Su Hyung\} and Park, \{Young Woo\} and Shin, \{Eui Cheol\}",

year = "2015",

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day = "30",

doi = "10.1016/j.bbrc.2015.06.172",

language = "English",

volume = "464",

pages = "512--518",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Activation of human natural killer cells by the soluble form of cellular prion protein

AU - Seong, Yeon Jae

AU - Sung, Pil Soo

AU - Jang, Young Soon

AU - Choi, Young Joon

AU - Park, Bum Chan

AU - Park, Su Hyung

AU - Park, Young Woo

AU - Shin, Eui Cheol

PY - 2015/7/30

Y1 - 2015/7/30

N2 - Cellular prion protein (PrPC) is widely expressed in various cell types, including cells of the immune system. However, the specific roles of PrPC in the immune system have not been clearly elucidated. In the present study, we investigated the effects of a soluble form of recombinant PrPC protein on human natural killer (NK) cells. Recombinant soluble PrPC protein was generated by fusion of human PrPC with the Fc portion of human IgG1 (PrPC-Fc). PrPC-Fc binds to the surface of human NK cells, particularly to CD56dim NK cells. PrPC-Fc induced the production of cytokines and chemokines and the degranulation of granzyme B from NK cells. In addition, PrPC-Fc facilitated the IL-15-induced proliferation of NK cells. PrPC-Fc induced phosphorylation of ERK-1/2 and JNK in NK cells, and inhibitors of the ERK or the JNK pathways abrogated PrPC-Fc-induced cytokine production in NK cells. In conclusion, the soluble form of recombinant PrPC-Fc protein activates human NK cells via the ERK and JNK signaling pathways.

AB - Cellular prion protein (PrPC) is widely expressed in various cell types, including cells of the immune system. However, the specific roles of PrPC in the immune system have not been clearly elucidated. In the present study, we investigated the effects of a soluble form of recombinant PrPC protein on human natural killer (NK) cells. Recombinant soluble PrPC protein was generated by fusion of human PrPC with the Fc portion of human IgG1 (PrPC-Fc). PrPC-Fc binds to the surface of human NK cells, particularly to CD56dim NK cells. PrPC-Fc induced the production of cytokines and chemokines and the degranulation of granzyme B from NK cells. In addition, PrPC-Fc facilitated the IL-15-induced proliferation of NK cells. PrPC-Fc induced phosphorylation of ERK-1/2 and JNK in NK cells, and inhibitors of the ERK or the JNK pathways abrogated PrPC-Fc-induced cytokine production in NK cells. In conclusion, the soluble form of recombinant PrPC-Fc protein activates human NK cells via the ERK and JNK signaling pathways.

KW - Activation

KW - Cellular prion protein

KW - Cytokines

KW - Natural killer cells

UR - https://www.scopus.com/pages/publications/84938418922

U2 - 10.1016/j.bbrc.2015.06.172

DO - 10.1016/j.bbrc.2015.06.172

M3 - Article

C2 - 26159919

AN - SCOPUS:84938418922

SN - 0006-291X

VL - 464

SP - 512

EP - 518

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Activation of human natural killer cells by the soluble form of cellular prion protein

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Keywords

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