AgNOR of human interphase cells in relation to acrocentric chromosomes

Wonbae Lee, Yonggoo Kim, Kyo Young Lee, Chang Suck Kang, Weonsun Lee, Kyung Soo Lee, Sang In Shim, Kyungja Han

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Using simultaneous detection of fluorescence in situ hybridization (FISH) to acrocentric chromosome centromeres and argyrophilic nucleolar organizer regions (AgNOR), we investigated the number of AgNOR and involvement pattern of acrocentric chromosomes in the nucleoli in various types of human interphase cells. The number of AgNOR of normal gastric mucosal epithelial cells was 2.27 ± 1.18 and was higher than that of lymphocytes (1.08 ± 0.28) and lower than that of gastric cancer (7.76 ± 3.21). The number of acrocentric chromosome centromere signals of normal gastric mucosal epithelial cells was higher than that of normal leukocytes (P < 0.000), and lower than that of gastric cancer (P < 0.000). The acrocentric chromosome centromere signals in the lymphocytes and neutrophils were only half of that expected for diploid cells, perhaps related to acrocentric chromosome association. The proportion of acrocentric chromosomes attached to AgNOR in gastric cancer (0.88 ± 0.22) was significantly higher than that of normal gastric mucosal epithelial cells (0.72 ± 0.35, P = 0.007). In conclusion, acrocentric chromosome association appears to be present in circulating leukocytes even in interphase. The number of AgNORs and proportion of acrocentric chromosomes involved in AgNORs in human interphase cells may vary according to cell types. This could play a significant role in rDNA transcription and determination of cell phenotype, including malignant change. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)14-18
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume113
Issue number1
DOIs
StatePublished - Aug 1999

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