Analysis of apparent diffusion coefficients of the brain in healthy controls: A comparison study between single-shot echo-planar imaging and read-out-segmented echo-planar imaging

Objective: To compare apparent diffusion coefficients (ADCs) of brain segments by using two diffusion-weighted imaging acquisition modes, single-shot echo-planar imaging (ss-EPI) and read-out-segmented echo-planar imaging (rs-EPI), and to assess their correlation and agreement in healthy controls. Materials and Methods: T2-weighted (T2W) images, rs-EPI, and ss-EPI of 30 healthy subjects were acquired using a 3T magnetic resonance scanner. The T2W images were co-registered to the rs-EPI and ss-EPI, which were then segmented into the gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) to generate masking templates. ADC maps of rs-EPI and ss-EPI were also segmented into the GM, WM, and CSF by using the generated templates. ADCs of rs-EPI and ss-EPI were compared using Student’s t tests and correlated using Pearson’s correlation coefficients. Bland-Altman plots were used to assess the agreement between acquisitions. Results: ADCs of rs-EPI and ss-EPI were significantly different in the GM (p < 0.001) and WM (p < 0.001). ADCs showed high agreement and correlation in the whole brain and CSF (r > 0.988; p < 0.001). ADC of the WM showed the least correlation (r = 0.894; p < 0.001), and ADCs of the WM and GM showed poor agreement. Pearson’s correlation equations for each brain segment were y = 1.1x-59.4 (GM), y = 1.45x-255 (WM), and y = 0.98x-63.5 (CSF), where x and y indicated ADCs of rs-EPI and ss-EPI, respectively. Conclusion: While ADCs of rs-EPI and ss-EPI showed high correlation and agreement in the whole brain and CSF, ADCs of the WM and GM showed significant differences and large variability, reflecting brain parenchymal inhomogeneity due to different regional microenvironments. ADCs of different acquisition methods should be interpreted carefully, especially in intra-individual comparisons.