Abstract
We have developed cellulose membrane (CM) from the native sea squirt skin since several years ago. The aim of this study is to analyze the basic chemical composition and fiber structure of this CM. To analyze the basic carbohydrate composition and its structures, HPAEC (High Performance Anion-Exchange Chromatography) was used. Elementary analysis by using element analyzer (EA 1110®, CE instruments Co.) and FE-SEM (Field Emission Scanning Electron Microscope), amino acid analysis by using amino acid analyzer (L-8800®, Hitachi Co.), transmission analysis with ATR-IR (Attenuated Total Reflection Infrared), surface chemical analysis by ESCA (Electron Spectroscopy for Chemical Analysis), and cellulose fibers analysis by SEM (Scanning Electron Microscopy) were all carried out. Glucose was the major constructive carbohydrate as 95%, and other minor fucose, arabinose and mannose were purified as 1∼2 % each. C=O functional group can be also checked in the organic and nonorganic synthetic materials, and surface chemical bonding energy of each carbon and oxygen was confirmed through C1s and O1s spectra. From these basic analysis, CM has pure carbohydrate polymer structures consisted of rigid outer surface and delicate inner surface, which will be able to be developed as the tissue regenerative barrier membrane in the medical scope.
| Original language | English |
|---|---|
| Pages (from-to) | 191-201 |
| Number of pages | 11 |
| Journal | Tissue Engineering and Regenerative Medicine |
| Volume | 7 |
| Issue number | 2 |
| State | Published - Mar 2010 |
Keywords
- Amino acid analysis
- Cellulose fibers analysis
- Cellulose membrane (CM)
- Elementary analysis
- Sea squirt
- Surface chemical analysis