Bioactive compounds isolated from defatted microalgal biomasses of botryococcus braunii and dunaliella tertiolecta showing a tyrosinase inhibitory activity

Aims: This study aims to elucidate the structural difference and biochemical properties of bioactive compounds of microalgal biomasses. Background: The structural difference and biochemical properties of bioactive compounds termed as Water-Soluble Macromolecules (WSMs) are interested in evaluating their biological activities. Methods: This study was performed to elucidate the structural difference and biochemical properties of bioactive compounds termed as Water-Soluble Macromolecules (WSMs) isolated from de-fatted microalgal biomasses of Botryococcus braunii and Dunaliella tertiolecta. Results: The compositional analysis of both WSMs revealed that WSM-Bb is a hetero-macro-molecule consisting of various monosaccharides, whereas WSM-Dt was characterized as a homo--macromolecule that mainly consists of glucose. Interestingly, WSM-Bb showed the significant ty-rosinase inhibitory activity with the increase of both the concentration and reaction time. Whereas there was no significant inhibitory activity observed by WSM-Dt. Conclusion: Inhibitory action of WSM-Bb toward both tyrosinase and tyrosine in either simultaneous or separate reaction may be mainly due to the physical affinity of WSM-Bb. These results em-phasize the identification of the primary components of these WSMs and their relevance with the antioxidant function.