Abstract
Background/Aim: 5-Fluorouracil (5-FU) is an anticancer drug commonly used to treat gastric cancer; however, continuous 5-FU chemotherapy causes drug resistance. Materials and Methods: We established five sublines of 5-FU-resistant AGS gastric cancer cells to investigate changes that may have occurred in the development of 5-FU resistance. Drug resistance to other chemotherapeutic reagents, proliferation, cell-cycle changes, and wound healing ability were assessed for each subline. Results: Retarded cell growth, G0/G1 phase arrest, upregulation of p57, and down-regulation of cyclin D1 were commonly observed in all five sublines. Resistance to paclitaxel and cisplatin was also observed in most of the sublines. Conclusion: Our data support the notion that G0/G1 arrest due to changes in p57 and cyclin D1 expression may confer drug resistance, while EMT seems non-essential to 5- FU resistance in AGS gastric carcinoma cells.
| Original language | English |
|---|---|
| Pages (from-to) | 3247-3254 |
| Number of pages | 8 |
| Journal | Anticancer Research |
| Volume | 40 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2020 |
Bibliographical note
Publisher Copyright:© 2020 International Institute of Anticancer Research. All rights reserved.
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- 5-FU
- Cell cycle
- Cyclin D1
- Drug resistance
- EMT
- Gastric cancer
- P57
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