TY - JOUR
T1 - Chronic saponin treatment attenuates damage to the pancreas in chronic alcohol-treated diabetic rats
AU - Choi, Mi Ran
AU - Kwak, Su Min
AU - Bang, Sol Hee
AU - Jeong, Jo Eun
AU - Kim, Dai Jin
N1 - Publisher Copyright:
© 2016
PY - 2017/10
Y1 - 2017/10
N2 - Background Chronic heavy alcohol consumption may raise the risk of developing type 2 diabetes mellitus. Saponins inhibit apoptosis of pancreatic islet cells and reduce lipid parameters. The present study was designed to investigate the effect of saponin on chronic ethanol-treated diabetic rats. Methods Long–Evans Tokushima Fatty (LETO) and Otsuka Long–Evans Tokushima Fatty (OLETF) rats were pair-fed a Lieber–DeCarli diet with and without 5% ethanol for 12 wks. Two weeks after starting the pair-feeding with the Lieber–DeCarli diet, intraperitoneal injection of saponin was performed for 10 wks. To perform the experiments, rats were divided as follows: LETO-Control (LC), LETO-Ethanol (LE), LETO-Ethanol-Saponin (LES), OLETF-Control (OC), OLETF-Ethanol (OE), and OLETF-Ethanol-Saponin (OES). Results The weights of epididymal and mesenteric fat tissue in LES and OES rats were the lightest from among the LETO and OLETF groups, respectively. The secretion of alanine aminotransferase and cholesterol in OES rats decreased significantly compared to their secretion in OC and OE rats, respectively. The islets of the pancreas in LE and OE rats showed clean, unclear, and smaller morphology compared to those of LC, LES, OC, and OES rats. In addition, the expression of insulin in the islets of the pancreas in LC, LES, OC, and OES rats was higher than in LE and OE rats. Conclusion Saponin may not only be helpful in alleviating the rapid progress of diabetes due to chronic alcohol consumption in diabetic patients, but may also show potential as an antidiabetic drug candidate for diabetic patients who chronically consume alcohol.
AB - Background Chronic heavy alcohol consumption may raise the risk of developing type 2 diabetes mellitus. Saponins inhibit apoptosis of pancreatic islet cells and reduce lipid parameters. The present study was designed to investigate the effect of saponin on chronic ethanol-treated diabetic rats. Methods Long–Evans Tokushima Fatty (LETO) and Otsuka Long–Evans Tokushima Fatty (OLETF) rats were pair-fed a Lieber–DeCarli diet with and without 5% ethanol for 12 wks. Two weeks after starting the pair-feeding with the Lieber–DeCarli diet, intraperitoneal injection of saponin was performed for 10 wks. To perform the experiments, rats were divided as follows: LETO-Control (LC), LETO-Ethanol (LE), LETO-Ethanol-Saponin (LES), OLETF-Control (OC), OLETF-Ethanol (OE), and OLETF-Ethanol-Saponin (OES). Results The weights of epididymal and mesenteric fat tissue in LES and OES rats were the lightest from among the LETO and OLETF groups, respectively. The secretion of alanine aminotransferase and cholesterol in OES rats decreased significantly compared to their secretion in OC and OE rats, respectively. The islets of the pancreas in LE and OE rats showed clean, unclear, and smaller morphology compared to those of LC, LES, OC, and OES rats. In addition, the expression of insulin in the islets of the pancreas in LC, LES, OC, and OES rats was higher than in LE and OE rats. Conclusion Saponin may not only be helpful in alleviating the rapid progress of diabetes due to chronic alcohol consumption in diabetic patients, but may also show potential as an antidiabetic drug candidate for diabetic patients who chronically consume alcohol.
KW - alcohol
KW - diabetes
KW - insulin
KW - pancreas
KW - saponin
UR - http://www.scopus.com/inward/record.url?scp=85005784441&partnerID=8YFLogxK
U2 - 10.1016/j.jgr.2016.09.002
DO - 10.1016/j.jgr.2016.09.002
M3 - Article
AN - SCOPUS:85005784441
SN - 1226-8453
VL - 41
SP - 503
EP - 512
JO - Journal of Ginseng Research
JF - Journal of Ginseng Research
IS - 4
ER -
