TY - JOUR
T1 - Coenzyme Q10 alleviates tacrolimus-induced mitochondrial dysfunction in kidney
AU - Yu, Ji Hyun
AU - Lim, Sun Woo
AU - Luo, Kang
AU - Cui, Sheng
AU - Quan, Yi
AU - Shin, Yoo Jin
AU - Lee, Kyung Eun
AU - Kim, Hong Lim
AU - Ko, Eun Jeong
AU - Chung, Byung Ha
AU - Kim, Ju Hwan
AU - Chung, Sang J.
AU - Yang, Chul Woo
N1 - Publisher Copyright:
© FASEB
PY - 2019/11/1
Y1 - 2019/11/1
N2 - The major side effect of tacrolimus (Tac) is nephrotoxicity. We studied whether supplementation of coenzyme Q10, (CoQ10) a potent antioxidant, can reduce Tac-induced nephrotoxicity via improving mitochondrial function. In an in vitro study, CoQ10 reduced the production of Tac-induced mitochondrial reactive oxygen species and abolished the loss of mitochondrial membrane potential in proximal tubular cell line. Assessment of mitochondrial function revealed that CoQ10 decreased oxygen consumption and mitochondrial respiration rate increased by Tac, suggesting improvement of mitochondrial function to synthesize ATP with CoQ10 treatment. The effect of the CoQ10 in vitro study was observed in an experimental model of chronic Tac-induced nephropathy. CoQ10 attenuated Tac-induced oxidative stress and was accompanied by function and histologic improvement. On electron microscopy, addition of CoQ10 increased not only the number but also the volume of mitochondria compared with Tac treatment only. Our data indicate that CoQ10 improves Tac-induced mitochondrial dysfunction in kidney. Supplementary CoQ10 treatment may be a promising approach to reduce Tac-induced nephrotoxicity.—Yu, J. H., Lim, S. W., Luo, K., Cui, S., Quan, Y., Shin, Y. J., Lee, K. E., Kim, H. L., Ko, E. J., Chung, B. H., Kim, J. H., Chung, S. J., Yang, C. W. Coenzyme Q10 alleviates tacrolimus-induced mitochondrial dysfunction in kidney. FASEB J. 33, 12288-12298 (2019). www.fasebj.org.
AB - The major side effect of tacrolimus (Tac) is nephrotoxicity. We studied whether supplementation of coenzyme Q10, (CoQ10) a potent antioxidant, can reduce Tac-induced nephrotoxicity via improving mitochondrial function. In an in vitro study, CoQ10 reduced the production of Tac-induced mitochondrial reactive oxygen species and abolished the loss of mitochondrial membrane potential in proximal tubular cell line. Assessment of mitochondrial function revealed that CoQ10 decreased oxygen consumption and mitochondrial respiration rate increased by Tac, suggesting improvement of mitochondrial function to synthesize ATP with CoQ10 treatment. The effect of the CoQ10 in vitro study was observed in an experimental model of chronic Tac-induced nephropathy. CoQ10 attenuated Tac-induced oxidative stress and was accompanied by function and histologic improvement. On electron microscopy, addition of CoQ10 increased not only the number but also the volume of mitochondria compared with Tac treatment only. Our data indicate that CoQ10 improves Tac-induced mitochondrial dysfunction in kidney. Supplementary CoQ10 treatment may be a promising approach to reduce Tac-induced nephrotoxicity.—Yu, J. H., Lim, S. W., Luo, K., Cui, S., Quan, Y., Shin, Y. J., Lee, K. E., Kim, H. L., Ko, E. J., Chung, B. H., Kim, J. H., Chung, S. J., Yang, C. W. Coenzyme Q10 alleviates tacrolimus-induced mitochondrial dysfunction in kidney. FASEB J. 33, 12288-12298 (2019). www.fasebj.org.
KW - 3D reconstruction
KW - nephrotoxicity
KW - reactive oxygen species
UR - https://www.scopus.com/pages/publications/85074379489
U2 - 10.1096/fj.201900386RR
DO - 10.1096/fj.201900386RR
M3 - Article
C2 - 31431058
AN - SCOPUS:85074379489
SN - 0892-6638
VL - 33
SP - 12288
EP - 12298
JO - FASEB Journal
JF - FASEB Journal
IS - 11
ER -