Comparative study of chronic postischemic pain models in mice: o-ring versus tie method

Yong Han Seo, Ye Won Gil, Gyuho Choe, Sung Hyun Lee, Sie Hyeon Yoo, Hue Jung Park

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background: The success rate for the production of animal models of chronic postischemia pain (CPIP) using an O-ring has yet to be improved in the study of complex regional pain syndrome-type I (CRPS-I), and producing a CPIP model is challenging, especially for mice. Objectives: We devised a new CPIP model with a higher success rate that induces ischemia for 3 hours by tying the hind limbs of mice with a rubber band, followed by reperfusion. Study Design: A randomized, controlled animal trial. Methods: Twenty-two male C57BL/6 mice were divided into a sham (n = 6), a ring (n = 8), and a tie group (n = 8). Anesthesia was induced using isoflurane. A precut O-ring was mounted on the upper left ankle in the sham group. A tight-fitting O-ring and a push-pull gauge manometer were mounted at the same location in the ring and tie groups, respectively. Reperfusion was induced 3 hours later. The thickness and circumference of the hind paws were measured before ischemia induction. Measurements were repeated 10 days after reperfusion. Mechanical allodynia was measured with a von Frey filament until 12 weeks after reperfusion. Results: The new tie model required 5 additional days until the onset of allodynia compared with the existing CPIP O-ring model. However, the successful induction rate of CPIP was higher in the tie group than in the ring group, and allodynia was maintained for over 30 days in the tie group. The ring and tie groups exhibited significantly high levels of tumor necrosis factor-alpha than those in the sham group. Limitations: First, we did not evaluate hyperalgesia, cold or heat allodynia. Second, we did not measure blood levels of inflammatory or antiinflammatory cytokines, and research on oxidative stress biomarkers such as isoprostane, 8-hydroxy-2’-deoxyguanosine (a marker of DNA oxidative damage), and malondialdehyde was not performed. Conclusions: The new CPIP tie model has a higher rate of successful induction than existing O-ring models for mice, with longer duration of mechanical allodynia. The model may reduce the number of animals sacrificed in CRPS-I research and could be useful for studying long-term effects of drugs.

Original languageEnglish
Pages (from-to)E51-E60
JournalPain Physician
Volume23
Issue number1
StatePublished - 2020

Bibliographical note

Funding Information:
Address Correspondence: Sie Hyeon Yoo, Md, PhD Soonchunhyang University Hospital Cheonan 31, Suncheonhyang 6-gil, Dongnam-gu, Cheonan-si, Chungcheongnam-do, Republic of Korea E-mail: [email protected] Disclaimer: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (grant number. 2017R1D1A1B03028360). Conflict of interest: Each author certifies that he or she, or a member of his or her immediate family, has no commercial association (i.e., consultancies, stock ownership, equity interest, patent/licensing arrangements, etc.) that might pose a conflict of interest in connection with the submitted manuscript.

Publisher Copyright:
© 2020, American Society of Interventional Pain Physicians. All rights reserved.

Keywords

  • Allodynia
  • CPIP
  • Hyperalgesia
  • Mouse
  • O-ring
  • Reperfusion
  • Rubber band

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