Comparison between subcutaneous injection of basic fibroblast growth factor-hydrogel and intracavernous injection of adipose-derived stem cells in a rat model of cavernous nerve injury

Jang Ho Bae; Kshitiz Raj Shrestha; Yong Hyun Park; In Gul Kim; Shuyu Piao; Ae Ryang Jung; Seung Hwan Jeon; Ki Dong Park; Ji Youl Lee

doi:10.1016/j.urology.2014.07.028

Comparison between subcutaneous injection of basic fibroblast growth factor-hydrogel and intracavernous injection of adipose-derived stem cells in a rat model of cavernous nerve injury

Jang Ho Bae, Kshitiz Raj Shrestha, Yong Hyun Park, In Gul Kim, Shuyu Piao, Ae Ryang Jung, Seung Hwan Jeon, Ki Dong Park, Ji Youl Lee

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Objective To compare the effects of subcutaneous penile injection of basic fibroblast growth factor (bFGF)-hydrogel and intracavernous injection of human adipose-derived stem cells (h-ADSCs) on improving erectile function in a rat model of cavernous nerve injury.

Materials and Methods Adult male Sprague-Dawley rats were randomly divided into 5 groups (n = 10 per group): age-matched control (normal group), bilateral cavernous nerve injury (BCNI group), penile subcutaneous injection of hydrogel after BCNI (hydrogel group), penile subcutaneous injection of bFGF-hydrogel after BCNI (bFGF-hydrogel group) and intracavernous injection of h-ADSCs after BCNI (ADSC group). Four weeks after the treatment, all rats underwent an erectile function test. Then, penile tissue was harvested for immunohistological analysis of bFGF, phalloidin, and cluster of differentiation (CD) 31. The cyclic guanosine monophosphate (cGMP) level of the corpus cavernosum was quantified by cGMP assay.

Results From the functional test and immunohistological result, we observed that bFGF-hydrogel and h-ADSCs injection significantly elevated intracavernous pressure. The evaluation of filamentous actin content, CD31 expression, and cGMP concentration in the corpus cavernosum were meaningfully increased in the bFGF-hydrogel and ADSC groups compared with BCNI group. The bFGF released from bFGF-hydrogel prevented smooth muscle atrophy. Moreover, bFGF expression was significantly increased in bFGF-hydrogel group.

Conclusion The subcutaneous injection of bFGF-hydrogel prevented smooth muscle atrophy, increased the intracavernous pressure, and improved erectile function like an intracavernous injection of h-ADSCs.

Original language	English
Pages (from-to)	1248.e1-1248.e7
Journal	Urology
Volume	84
Issue number	5
DOIs	https://doi.org/10.1016/j.urology.2014.07.028
State	Published - 1 Nov 2014

Bibliographical note

Funding Information:
Funding Support: This work was partially supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government by the Ministry of Science , ICT and Future Planning (No. 2011-0030075 ), and partially by the Basic Science Research Program through the NRF funded by the Ministry of Science, ICT and Future Planning ( NRF-2014R1A2A2A01004955 ).

Publisher Copyright:
© 2014 Elsevier Inc.

Access to Document

10.1016/j.urology.2014.07.028

Cite this

Bae, J. H., Shrestha, K. R., Park, Y. H., Kim, I. G., Piao, S., Jung, A. R., Jeon, S. H., Park, K. D., & Lee, J. Y. (2014). Comparison between subcutaneous injection of basic fibroblast growth factor-hydrogel and intracavernous injection of adipose-derived stem cells in a rat model of cavernous nerve injury. Urology, 84(5), 1248.e1-1248.e7. https://doi.org/10.1016/j.urology.2014.07.028

@article{96482add689249338eee44a225476483,

title = "Comparison between subcutaneous injection of basic fibroblast growth factor-hydrogel and intracavernous injection of adipose-derived stem cells in a rat model of cavernous nerve injury",

abstract = "Objective To compare the effects of subcutaneous penile injection of basic fibroblast growth factor (bFGF)-hydrogel and intracavernous injection of human adipose-derived stem cells (h-ADSCs) on improving erectile function in a rat model of cavernous nerve injury.Materials and Methods Adult male Sprague-Dawley rats were randomly divided into 5 groups (n = 10 per group): age-matched control (normal group), bilateral cavernous nerve injury (BCNI group), penile subcutaneous injection of hydrogel after BCNI (hydrogel group), penile subcutaneous injection of bFGF-hydrogel after BCNI (bFGF-hydrogel group) and intracavernous injection of h-ADSCs after BCNI (ADSC group). Four weeks after the treatment, all rats underwent an erectile function test. Then, penile tissue was harvested for immunohistological analysis of bFGF, phalloidin, and cluster of differentiation (CD) 31. The cyclic guanosine monophosphate (cGMP) level of the corpus cavernosum was quantified by cGMP assay.Results From the functional test and immunohistological result, we observed that bFGF-hydrogel and h-ADSCs injection significantly elevated intracavernous pressure. The evaluation of filamentous actin content, CD31 expression, and cGMP concentration in the corpus cavernosum were meaningfully increased in the bFGF-hydrogel and ADSC groups compared with BCNI group. The bFGF released from bFGF-hydrogel prevented smooth muscle atrophy. Moreover, bFGF expression was significantly increased in bFGF-hydrogel group.Conclusion The subcutaneous injection of bFGF-hydrogel prevented smooth muscle atrophy, increased the intracavernous pressure, and improved erectile function like an intracavernous injection of h-ADSCs.",

author = "Bae, \{Jang Ho\} and Shrestha, \{Kshitiz Raj\} and Park, \{Yong Hyun\} and Kim, \{In Gul\} and Shuyu Piao and Jung, \{Ae Ryang\} and Jeon, \{Seung Hwan\} and Park, \{Ki Dong\} and Lee, \{Ji Youl\}",

note = "Funding Information: Funding Support: This work was partially supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government by the Ministry of Science , ICT and Future Planning (No. 2011-0030075 ), and partially by the Basic Science Research Program through the NRF funded by the Ministry of Science, ICT and Future Planning ( NRF-2014R1A2A2A01004955 ). Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",

year = "2014",

month = nov,

day = "1",

doi = "10.1016/j.urology.2014.07.028",

language = "English",

volume = "84",

pages = "1248.e1--1248.e7",

journal = "Urology",

issn = "0090-4295",

publisher = "Elsevier Inc.",

number = "5",

}

Bae, JH, Shrestha, KR, Park, YH, Kim, IG, Piao, S, Jung, AR, Jeon, SH, Park, KD & Lee, JY 2014, 'Comparison between subcutaneous injection of basic fibroblast growth factor-hydrogel and intracavernous injection of adipose-derived stem cells in a rat model of cavernous nerve injury', Urology, vol. 84, no. 5, pp. 1248.e1-1248.e7. https://doi.org/10.1016/j.urology.2014.07.028

Comparison between subcutaneous injection of basic fibroblast growth factor-hydrogel and intracavernous injection of adipose-derived stem cells in a rat model of cavernous nerve injury. / Bae, Jang Ho; Shrestha, Kshitiz Raj; Park, Yong Hyun et al.
In: Urology, Vol. 84, No. 5, 01.11.2014, p. 1248.e1-1248.e7.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comparison between subcutaneous injection of basic fibroblast growth factor-hydrogel and intracavernous injection of adipose-derived stem cells in a rat model of cavernous nerve injury

AU - Bae, Jang Ho

AU - Shrestha, Kshitiz Raj

AU - Park, Yong Hyun

AU - Kim, In Gul

AU - Piao, Shuyu

AU - Jung, Ae Ryang

AU - Jeon, Seung Hwan

AU - Park, Ki Dong

AU - Lee, Ji Youl

N1 - Funding Information: Funding Support: This work was partially supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government by the Ministry of Science , ICT and Future Planning (No. 2011-0030075 ), and partially by the Basic Science Research Program through the NRF funded by the Ministry of Science, ICT and Future Planning ( NRF-2014R1A2A2A01004955 ). Publisher Copyright: © 2014 Elsevier Inc.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Objective To compare the effects of subcutaneous penile injection of basic fibroblast growth factor (bFGF)-hydrogel and intracavernous injection of human adipose-derived stem cells (h-ADSCs) on improving erectile function in a rat model of cavernous nerve injury.Materials and Methods Adult male Sprague-Dawley rats were randomly divided into 5 groups (n = 10 per group): age-matched control (normal group), bilateral cavernous nerve injury (BCNI group), penile subcutaneous injection of hydrogel after BCNI (hydrogel group), penile subcutaneous injection of bFGF-hydrogel after BCNI (bFGF-hydrogel group) and intracavernous injection of h-ADSCs after BCNI (ADSC group). Four weeks after the treatment, all rats underwent an erectile function test. Then, penile tissue was harvested for immunohistological analysis of bFGF, phalloidin, and cluster of differentiation (CD) 31. The cyclic guanosine monophosphate (cGMP) level of the corpus cavernosum was quantified by cGMP assay.Results From the functional test and immunohistological result, we observed that bFGF-hydrogel and h-ADSCs injection significantly elevated intracavernous pressure. The evaluation of filamentous actin content, CD31 expression, and cGMP concentration in the corpus cavernosum were meaningfully increased in the bFGF-hydrogel and ADSC groups compared with BCNI group. The bFGF released from bFGF-hydrogel prevented smooth muscle atrophy. Moreover, bFGF expression was significantly increased in bFGF-hydrogel group.Conclusion The subcutaneous injection of bFGF-hydrogel prevented smooth muscle atrophy, increased the intracavernous pressure, and improved erectile function like an intracavernous injection of h-ADSCs.

AB - Objective To compare the effects of subcutaneous penile injection of basic fibroblast growth factor (bFGF)-hydrogel and intracavernous injection of human adipose-derived stem cells (h-ADSCs) on improving erectile function in a rat model of cavernous nerve injury.Materials and Methods Adult male Sprague-Dawley rats were randomly divided into 5 groups (n = 10 per group): age-matched control (normal group), bilateral cavernous nerve injury (BCNI group), penile subcutaneous injection of hydrogel after BCNI (hydrogel group), penile subcutaneous injection of bFGF-hydrogel after BCNI (bFGF-hydrogel group) and intracavernous injection of h-ADSCs after BCNI (ADSC group). Four weeks after the treatment, all rats underwent an erectile function test. Then, penile tissue was harvested for immunohistological analysis of bFGF, phalloidin, and cluster of differentiation (CD) 31. The cyclic guanosine monophosphate (cGMP) level of the corpus cavernosum was quantified by cGMP assay.Results From the functional test and immunohistological result, we observed that bFGF-hydrogel and h-ADSCs injection significantly elevated intracavernous pressure. The evaluation of filamentous actin content, CD31 expression, and cGMP concentration in the corpus cavernosum were meaningfully increased in the bFGF-hydrogel and ADSC groups compared with BCNI group. The bFGF released from bFGF-hydrogel prevented smooth muscle atrophy. Moreover, bFGF expression was significantly increased in bFGF-hydrogel group.Conclusion The subcutaneous injection of bFGF-hydrogel prevented smooth muscle atrophy, increased the intracavernous pressure, and improved erectile function like an intracavernous injection of h-ADSCs.

UR - https://www.scopus.com/pages/publications/84908318212

U2 - 10.1016/j.urology.2014.07.028

DO - 10.1016/j.urology.2014.07.028

M3 - Article

C2 - 25443945

AN - SCOPUS:84908318212

SN - 0090-4295

VL - 84

SP - 1248.e1-1248.e7

JO - Urology

JF - Urology

IS - 5

ER -

Comparison between subcutaneous injection of basic fibroblast growth factor-hydrogel and intracavernous injection of adipose-derived stem cells in a rat model of cavernous nerve injury

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this