Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems for identification of clinical bacteria and yeasts

Jin Jung; Soo Young Kim; Yeon Joon Park; Jaewoong Lee; Hyun Soo Suk; Sung Il Ha; Jeong Seob Shin; Kang Gyun Park; Yeongsic Kim

doi:10.1016/j.jiac.2020.08.004

Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems for identification of clinical bacteria and yeasts

Jin Jung, Soo Young Kim, Yeon Joon Park, Jaewoong Lee, Hyun Soo Suk, Sung Il Ha, Jeong Seob Shin, Kang Gyun Park, Yeongsic Kim

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The ASTA MicroIDSys system (ASTA, Suwon, Korea) is a newly developed Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for identification of microorganisms. We compared the performance of the ASTA MicroIDSys system with that of the VITEK MS system (bioMérieux, Marcy l'Etoile, France) for identifying clinical microorganisms. A total 2055 isolates including 1910 bacteria and 145 yeasts were tested. Among them, the VITEK MS correctly identified 1999 (97.3%) isolates to species level and 26 (1.3%) to the genus level. The ASTA MicroIDSys correctly identified 1988 (96.7%) isolates to species level and 28 (1.4%) to the genus level. The VITEK MS and ASTA MicroIDSys misidentified one isolate and four (0.2%) isolates, respectively, and provided no identification for 29 (1.4%) and 35 (1.7%) isolates, respectively. The performance of the ASTA MicroIDSys was comparable to that of the VITEK MS for identification of clinically relevant bacterial and yeast isolates.

Original language	English
Pages (from-to)	1328-1333
Number of pages	6
Journal	Journal of Infection and Chemotherapy
Volume	26
Issue number	12
DOIs	https://doi.org/10.1016/j.jiac.2020.08.004
State	Published - Dec 2020

Bibliographical note

Funding Information:
This work was supported by the Ministry of Health & Welfare, Republic of Korea (grant number: HI18C-2318-020019).

Funding Information:
This work was supported by the Ministry of Health & Welfare, Republic of Korea (grant number: HI18C-2318-020019 ).

Publisher Copyright:
© 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases

Keywords

ASTA MicroIDSys
Bacteria
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
VITEK MS
Yeast

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jiac.2020.08.004

Cite this

Jung, J., Kim, S. Y., Park, Y. J., Lee, J., Suk, H. S., Ha, S. I., Shin, J. S., Park, K. G., & Kim, Y. (2020). Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems for identification of clinical bacteria and yeasts. Journal of Infection and Chemotherapy, 26(12), 1328-1333. https://doi.org/10.1016/j.jiac.2020.08.004

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title = "Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems for identification of clinical bacteria and yeasts",

abstract = "The ASTA MicroIDSys system (ASTA, Suwon, Korea) is a newly developed Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for identification of microorganisms. We compared the performance of the ASTA MicroIDSys system with that of the VITEK MS system (bioM{\'e}rieux, Marcy l'Etoile, France) for identifying clinical microorganisms. A total 2055 isolates including 1910 bacteria and 145 yeasts were tested. Among them, the VITEK MS correctly identified 1999 (97.3%) isolates to species level and 26 (1.3%) to the genus level. The ASTA MicroIDSys correctly identified 1988 (96.7%) isolates to species level and 28 (1.4%) to the genus level. The VITEK MS and ASTA MicroIDSys misidentified one isolate and four (0.2%) isolates, respectively, and provided no identification for 29 (1.4%) and 35 (1.7%) isolates, respectively. The performance of the ASTA MicroIDSys was comparable to that of the VITEK MS for identification of clinically relevant bacterial and yeast isolates.",

keywords = "ASTA MicroIDSys, Bacteria, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, VITEK MS, Yeast",

author = "Jin Jung and Kim, {Soo Young} and Park, {Yeon Joon} and Jaewoong Lee and Suk, {Hyun Soo} and Ha, {Sung Il} and Shin, {Jeong Seob} and Park, {Kang Gyun} and Yeongsic Kim",

note = "Funding Information: This work was supported by the Ministry of Health & Welfare, Republic of Korea (grant number: HI18C-2318-020019). Funding Information: This work was supported by the Ministry of Health & Welfare, Republic of Korea (grant number: HI18C-2318-020019 ). Publisher Copyright: {\textcopyright} 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases",

year = "2020",

month = dec,

doi = "10.1016/j.jiac.2020.08.004",

language = "English",

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Jung, J, Kim, SY, Park, YJ, Lee, J, Suk, HS, Ha, SI, Shin, JS, Park, KG & Kim, Y 2020, 'Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems for identification of clinical bacteria and yeasts', Journal of Infection and Chemotherapy, vol. 26, no. 12, pp. 1328-1333. https://doi.org/10.1016/j.jiac.2020.08.004

Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems for identification of clinical bacteria and yeasts. / Jung, Jin; Kim, Soo Young; Park, Yeon Joon et al.
In: Journal of Infection and Chemotherapy, Vol. 26, No. 12, 12.2020, p. 1328-1333.

Research output: Contribution to journal › Article › peer-review

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T1 - Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems for identification of clinical bacteria and yeasts

AU - Jung, Jin

AU - Kim, Soo Young

AU - Park, Yeon Joon

AU - Lee, Jaewoong

AU - Suk, Hyun Soo

AU - Ha, Sung Il

AU - Shin, Jeong Seob

AU - Park, Kang Gyun

AU - Kim, Yeongsic

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N2 - The ASTA MicroIDSys system (ASTA, Suwon, Korea) is a newly developed Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for identification of microorganisms. We compared the performance of the ASTA MicroIDSys system with that of the VITEK MS system (bioMérieux, Marcy l'Etoile, France) for identifying clinical microorganisms. A total 2055 isolates including 1910 bacteria and 145 yeasts were tested. Among them, the VITEK MS correctly identified 1999 (97.3%) isolates to species level and 26 (1.3%) to the genus level. The ASTA MicroIDSys correctly identified 1988 (96.7%) isolates to species level and 28 (1.4%) to the genus level. The VITEK MS and ASTA MicroIDSys misidentified one isolate and four (0.2%) isolates, respectively, and provided no identification for 29 (1.4%) and 35 (1.7%) isolates, respectively. The performance of the ASTA MicroIDSys was comparable to that of the VITEK MS for identification of clinically relevant bacterial and yeast isolates.

AB - The ASTA MicroIDSys system (ASTA, Suwon, Korea) is a newly developed Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for identification of microorganisms. We compared the performance of the ASTA MicroIDSys system with that of the VITEK MS system (bioMérieux, Marcy l'Etoile, France) for identifying clinical microorganisms. A total 2055 isolates including 1910 bacteria and 145 yeasts were tested. Among them, the VITEK MS correctly identified 1999 (97.3%) isolates to species level and 26 (1.3%) to the genus level. The ASTA MicroIDSys correctly identified 1988 (96.7%) isolates to species level and 28 (1.4%) to the genus level. The VITEK MS and ASTA MicroIDSys misidentified one isolate and four (0.2%) isolates, respectively, and provided no identification for 29 (1.4%) and 35 (1.7%) isolates, respectively. The performance of the ASTA MicroIDSys was comparable to that of the VITEK MS for identification of clinically relevant bacterial and yeast isolates.

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KW - Bacteria

KW - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

KW - VITEK MS

KW - Yeast

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SN - 1341-321X

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ER -

Comparison of the ASTA MicroIDSys and VITEK MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems for identification of clinical bacteria and yeasts

Abstract

Bibliographical note

Keywords

UN SDGs

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