Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer

Hyeran Shim; Kiwon Jang; Yeong Hak Bang; Hoang Bao Khanh Chu; Jisun Kang; Jin Young Lee; Sheehyun Cho; Hong Seok Lee; Jongbum Jeon; Taeyeon Hwang; Soobok Joe; Jinyeong Lim; Ji Hye Choi; Eun Hye Joo; Kyunghee Park; Ji Hwan Moon; Kyung Yeon Han; Yourae Hong; Woo Yong Lee; Hee Cheol Kim; Seong Hyeon Yun; Yong Beom Cho; Yoon Ah Park; Jung Wook Huh; Jung Kyong Shin; Dae Hee Pyo; Hyekyung Hong; Hae Ock Lee; Woong Yang Park; Jin Ok Yang; Young Joon Kim

doi:10.5483/BMBRep.2023-0093

Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer

Hyeran Shim, Kiwon Jang, Yeong Hak Bang, Hoang Bao Khanh Chu, Jisun Kang, Jin Young Lee, Sheehyun Cho, Hong Seok Lee, Jongbum Jeon, Taeyeon Hwang, Soobok Joe, Jinyeong Lim, Ji Hye Choi, Eun Hye Joo, Kyunghee Park, Ji Hwan Moon, Kyung Yeon Han, Yourae Hong, Woo Yong Lee, Hee Cheol KimSeong Hyeon Yun, Yong Beom Cho, Yoon Ah Park, Jung Wook Huh, Jung Kyong Shin, Dae Hee Pyo, Hyekyung Hong, Hae Ock Lee, Woong Yang Park, Jin Ok Yang, Young Joon Kim

Department of Microbiology

Research output: Contribution to journal › Article › peer-review

Abstract

Alterations in DNA methylation play an important pathophysiological role in the development and progression of colorectal cancer. We comprehensively profiled DNA methylation alterations in 165 Korean patients with colorectal cancer (CRC), and conducted an in-depth investigation of cancer-specific methylation patterns. Our analysis of the tumor samples revealed a significant presence of hypomethylated probes, primarily within the gene body regions; few hypermethylated sites were observed, which were mostly enriched in promoter-like and CpG island regions. The CpG Island Methylator Phenotype-High (CIMP-H) exhibited notable enrichment of microsatellite instability-high (MSI-H). Additionally, our findings indicated a significant correlation between methylation of the MLH1 gene and MSI-H status. Furthermore, we found that the CIMP-H had a higher tendency to affect the right-side of the colon tissues and was slightly more prevalent among older patients. Through our methylome profile analysis, we successfully verified the methylation patterns and clinical characteristics of Korean patients with CRC.

Original language	English
Pages (from-to)	110-115
Number of pages	6
Journal	BMB Reports
Volume	57
Issue number	2
DOIs	https://doi.org/10.5483/BMBRep.2023-0093
State	Published - 2024

Bibliographical note

Publisher Copyright:
© 2024 by the The Korean Society for Biochemistry and Molecular Biology. All Rights Reserved.

Keywords

Colorectal cancer
CpG island methylator phenotype (CIMP)
DNA methylation
MutL homolog 1 (MLH1)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5483/BMBRep.2023-0093

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Shim, H., Jang, K., Bang, Y. H., Chu, H. B. K., Kang, J., Lee, J. Y., Cho, S., Lee, H. S., Jeon, J., Hwang, T., Joe, S., Lim, J., Choi, J. H., Joo, E. H., Park, K., Moon, J. H., Han, K. Y., Hong, Y., Lee, W. Y., ... Kim, Y. J. (2024). Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer. BMB Reports, 57(2), 110-115. https://doi.org/10.5483/BMBRep.2023-0093

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title = "Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer",

abstract = "Alterations in DNA methylation play an important pathophysiological role in the development and progression of colorectal cancer. We comprehensively profiled DNA methylation alterations in 165 Korean patients with colorectal cancer (CRC), and conducted an in-depth investigation of cancer-specific methylation patterns. Our analysis of the tumor samples revealed a significant presence of hypomethylated probes, primarily within the gene body regions; few hypermethylated sites were observed, which were mostly enriched in promoter-like and CpG island regions. The CpG Island Methylator Phenotype-High (CIMP-H) exhibited notable enrichment of microsatellite instability-high (MSI-H). Additionally, our findings indicated a significant correlation between methylation of the MLH1 gene and MSI-H status. Furthermore, we found that the CIMP-H had a higher tendency to affect the right-side of the colon tissues and was slightly more prevalent among older patients. Through our methylome profile analysis, we successfully verified the methylation patterns and clinical characteristics of Korean patients with CRC.",

keywords = "Colorectal cancer, CpG island methylator phenotype (CIMP), DNA methylation, MutL homolog 1 (MLH1)",

author = "Hyeran Shim and Kiwon Jang and Bang, {Yeong Hak} and Chu, {Hoang Bao Khanh} and Jisun Kang and Lee, {Jin Young} and Sheehyun Cho and Lee, {Hong Seok} and Jongbum Jeon and Taeyeon Hwang and Soobok Joe and Jinyeong Lim and Choi, {Ji Hye} and Joo, {Eun Hye} and Kyunghee Park and Moon, {Ji Hwan} and Han, {Kyung Yeon} and Yourae Hong and Lee, {Woo Yong} and Kim, {Hee Cheol} and Yun, {Seong Hyeon} and Cho, {Yong Beom} and Park, {Yoon Ah} and Huh, {Jung Wook} and Shin, {Jung Kyong} and Pyo, {Dae Hee} and Hyekyung Hong and Lee, {Hae Ock} and Park, {Woong Yang} and Yang, {Jin Ok} and Kim, {Young Joon}",

year = "2024",

doi = "10.5483/BMBRep.2023-0093",

language = "English",

volume = "57",

pages = "110--115",

journal = "BMB Reports",

issn = "1976-6696",

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Shim, H, Jang, K, Bang, YH, Chu, HBK, Kang, J, Lee, JY, Cho, S, Lee, HS, Jeon, J, Hwang, T, Joe, S, Lim, J, Choi, JH, Joo, EH, Park, K, Moon, JH, Han, KY, Hong, Y, Lee, WY, Kim, HC, Yun, SH, Cho, YB, Park, YA, Huh, JW, Shin, JK, Pyo, DH, Hong, H, Lee, HO, Park, WY, Yang, JO & Kim, YJ 2024, 'Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer', BMB Reports, vol. 57, no. 2, pp. 110-115. https://doi.org/10.5483/BMBRep.2023-0093

TY - JOUR

T1 - Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer

AU - Shim, Hyeran

AU - Jang, Kiwon

AU - Bang, Yeong Hak

AU - Chu, Hoang Bao Khanh

AU - Kang, Jisun

AU - Lee, Jin Young

AU - Cho, Sheehyun

AU - Lee, Hong Seok

AU - Jeon, Jongbum

AU - Hwang, Taeyeon

AU - Joe, Soobok

AU - Lim, Jinyeong

AU - Choi, Ji Hye

AU - Joo, Eun Hye

AU - Park, Kyunghee

AU - Moon, Ji Hwan

AU - Han, Kyung Yeon

AU - Hong, Yourae

AU - Lee, Woo Yong

AU - Kim, Hee Cheol

AU - Yun, Seong Hyeon

AU - Cho, Yong Beom

AU - Park, Yoon Ah

AU - Huh, Jung Wook

AU - Shin, Jung Kyong

AU - Pyo, Dae Hee

AU - Hong, Hyekyung

AU - Lee, Hae Ock

AU - Park, Woong Yang

AU - Yang, Jin Ok

AU - Kim, Young Joon

PY - 2024

Y1 - 2024

N2 - Alterations in DNA methylation play an important pathophysiological role in the development and progression of colorectal cancer. We comprehensively profiled DNA methylation alterations in 165 Korean patients with colorectal cancer (CRC), and conducted an in-depth investigation of cancer-specific methylation patterns. Our analysis of the tumor samples revealed a significant presence of hypomethylated probes, primarily within the gene body regions; few hypermethylated sites were observed, which were mostly enriched in promoter-like and CpG island regions. The CpG Island Methylator Phenotype-High (CIMP-H) exhibited notable enrichment of microsatellite instability-high (MSI-H). Additionally, our findings indicated a significant correlation between methylation of the MLH1 gene and MSI-H status. Furthermore, we found that the CIMP-H had a higher tendency to affect the right-side of the colon tissues and was slightly more prevalent among older patients. Through our methylome profile analysis, we successfully verified the methylation patterns and clinical characteristics of Korean patients with CRC.

AB - Alterations in DNA methylation play an important pathophysiological role in the development and progression of colorectal cancer. We comprehensively profiled DNA methylation alterations in 165 Korean patients with colorectal cancer (CRC), and conducted an in-depth investigation of cancer-specific methylation patterns. Our analysis of the tumor samples revealed a significant presence of hypomethylated probes, primarily within the gene body regions; few hypermethylated sites were observed, which were mostly enriched in promoter-like and CpG island regions. The CpG Island Methylator Phenotype-High (CIMP-H) exhibited notable enrichment of microsatellite instability-high (MSI-H). Additionally, our findings indicated a significant correlation between methylation of the MLH1 gene and MSI-H status. Furthermore, we found that the CIMP-H had a higher tendency to affect the right-side of the colon tissues and was slightly more prevalent among older patients. Through our methylome profile analysis, we successfully verified the methylation patterns and clinical characteristics of Korean patients with CRC.

KW - Colorectal cancer

KW - CpG island methylator phenotype (CIMP)

KW - DNA methylation

KW - MutL homolog 1 (MLH1)

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U2 - 10.5483/BMBRep.2023-0093

DO - 10.5483/BMBRep.2023-0093

M3 - Article

C2 - 37605617

AN - SCOPUS:85186749683

SN - 1976-6696

VL - 57

SP - 110

EP - 115

JO - BMB Reports

JF - BMB Reports

IS - 2

ER -

Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer

Abstract

Bibliographical note

Keywords

UN SDGs

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