CpG-oligodeoxynucleotide protects immune cells from γ-irradiation- induced cell death

Wern Joo Sohn; Keun Wook Lee; Soo Young Choi; Eunkyung Chung; Younghee Lee; Tae Yoon Kim; Suk Kyeong Lee; Yong Kyoung Choe; Jeung Hoon Lee; Doo Sik Kim; Hyung Joo Kwon

doi:10.1016/j.molimm.2005.07.020

CpG-oligodeoxynucleotide protects immune cells from γ-irradiation- induced cell death

Wern Joo Sohn, Keun Wook Lee, Soo Young Choi, Eunkyung Chung, Younghee Lee, Tae Yoon Kim, Suk Kyeong Lee, Yong Kyoung Choe, Jeung Hoon Lee, Doo Sik Kim, Hyung Joo Kwon

Department of Biomedicine & Health Science

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Synthetic oligodeoxynucleotides (CpG-ODNs) and bacterial DNA containing unmethylated CpG dinucleotides in the context of particular base sequences (CpG motifs) are known to inhibit anti-IgM-induced growth arrest and apoptosis of WHEI 231 B lymphocytes, and spontaneous apoptosis of mature spleen B cells in a sequence-specific fashion of the CpG-ODN. Here we report that CpG-ODN protects from the cell death induced by γ-irradiation of primary mouse spleen cells as well as mouse RAW 264.7 macrophage cells and human RPMI 8226 B cells. Experimental results showed that CpG-ODN promotes growth of the cells, and protects the cells from γ-irradiation-induced cell death accompanying Bcl-xS/L and Bcl-2 upregulation. Furthermore, survival of macrophages was enhanced when splenocytes were pretreated with CpG-ODN. Our results suggest the potential application of CpG-ODNs for more efficient cancer radiotherapy by enhancing survival of normal immune cells after radiation damage.

Original language	English
Pages (from-to)	1163-1171
Number of pages	9
Journal	Molecular Immunology
Volume	43
Issue number	8
DOIs	https://doi.org/10.1016/j.molimm.2005.07.020
State	Published - Mar 2006

Bibliographical note

Funding Information:
This work was supported by the grant from Korea science and engineering foundation (KOSEF) and ministry of science and technology (MOST) national nuclear technology program M20376080001-04B0509 and from KRIBB research initiative program. Kwon, H.-J. was supported by the grant from Hallym University.

Keywords

Apoptosis
B cells
CpG-ODN
Macrophages
γ-Irradiation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.molimm.2005.07.020

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title = "CpG-oligodeoxynucleotide protects immune cells from γ-irradiation- induced cell death",

abstract = "Synthetic oligodeoxynucleotides (CpG-ODNs) and bacterial DNA containing unmethylated CpG dinucleotides in the context of particular base sequences (CpG motifs) are known to inhibit anti-IgM-induced growth arrest and apoptosis of WHEI 231 B lymphocytes, and spontaneous apoptosis of mature spleen B cells in a sequence-specific fashion of the CpG-ODN. Here we report that CpG-ODN protects from the cell death induced by γ-irradiation of primary mouse spleen cells as well as mouse RAW 264.7 macrophage cells and human RPMI 8226 B cells. Experimental results showed that CpG-ODN promotes growth of the cells, and protects the cells from γ-irradiation-induced cell death accompanying Bcl-xS/L and Bcl-2 upregulation. Furthermore, survival of macrophages was enhanced when splenocytes were pretreated with CpG-ODN. Our results suggest the potential application of CpG-ODNs for more efficient cancer radiotherapy by enhancing survival of normal immune cells after radiation damage.",

keywords = "Apoptosis, B cells, CpG-ODN, Macrophages, γ-Irradiation",

author = "Sohn, {Wern Joo} and Lee, {Keun Wook} and Choi, {Soo Young} and Eunkyung Chung and Younghee Lee and Kim, {Tae Yoon} and Lee, {Suk Kyeong} and Choe, {Yong Kyoung} and Lee, {Jeung Hoon} and Kim, {Doo Sik} and Kwon, {Hyung Joo}",

note = "Funding Information: This work was supported by the grant from Korea science and engineering foundation (KOSEF) and ministry of science and technology (MOST) national nuclear technology program M20376080001-04B0509 and from KRIBB research initiative program. Kwon, H.-J. was supported by the grant from Hallym University.",

year = "2006",

month = mar,

doi = "10.1016/j.molimm.2005.07.020",

language = "English",

volume = "43",

pages = "1163--1171",

journal = "Molecular Immunology",

issn = "0161-5890",

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TY - JOUR

T1 - CpG-oligodeoxynucleotide protects immune cells from γ-irradiation- induced cell death

AU - Sohn, Wern Joo

AU - Lee, Keun Wook

AU - Choi, Soo Young

AU - Chung, Eunkyung

AU - Lee, Younghee

AU - Kim, Tae Yoon

AU - Lee, Suk Kyeong

AU - Choe, Yong Kyoung

AU - Lee, Jeung Hoon

AU - Kim, Doo Sik

AU - Kwon, Hyung Joo

N1 - Funding Information: This work was supported by the grant from Korea science and engineering foundation (KOSEF) and ministry of science and technology (MOST) national nuclear technology program M20376080001-04B0509 and from KRIBB research initiative program. Kwon, H.-J. was supported by the grant from Hallym University.

PY - 2006/3

Y1 - 2006/3

N2 - Synthetic oligodeoxynucleotides (CpG-ODNs) and bacterial DNA containing unmethylated CpG dinucleotides in the context of particular base sequences (CpG motifs) are known to inhibit anti-IgM-induced growth arrest and apoptosis of WHEI 231 B lymphocytes, and spontaneous apoptosis of mature spleen B cells in a sequence-specific fashion of the CpG-ODN. Here we report that CpG-ODN protects from the cell death induced by γ-irradiation of primary mouse spleen cells as well as mouse RAW 264.7 macrophage cells and human RPMI 8226 B cells. Experimental results showed that CpG-ODN promotes growth of the cells, and protects the cells from γ-irradiation-induced cell death accompanying Bcl-xS/L and Bcl-2 upregulation. Furthermore, survival of macrophages was enhanced when splenocytes were pretreated with CpG-ODN. Our results suggest the potential application of CpG-ODNs for more efficient cancer radiotherapy by enhancing survival of normal immune cells after radiation damage.

AB - Synthetic oligodeoxynucleotides (CpG-ODNs) and bacterial DNA containing unmethylated CpG dinucleotides in the context of particular base sequences (CpG motifs) are known to inhibit anti-IgM-induced growth arrest and apoptosis of WHEI 231 B lymphocytes, and spontaneous apoptosis of mature spleen B cells in a sequence-specific fashion of the CpG-ODN. Here we report that CpG-ODN protects from the cell death induced by γ-irradiation of primary mouse spleen cells as well as mouse RAW 264.7 macrophage cells and human RPMI 8226 B cells. Experimental results showed that CpG-ODN promotes growth of the cells, and protects the cells from γ-irradiation-induced cell death accompanying Bcl-xS/L and Bcl-2 upregulation. Furthermore, survival of macrophages was enhanced when splenocytes were pretreated with CpG-ODN. Our results suggest the potential application of CpG-ODNs for more efficient cancer radiotherapy by enhancing survival of normal immune cells after radiation damage.

KW - Apoptosis

KW - B cells

KW - CpG-ODN

KW - Macrophages

KW - γ-Irradiation

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ER -

CpG-oligodeoxynucleotide protects immune cells from γ-irradiation- induced cell death

Abstract

Bibliographical note

Keywords

UN SDGs

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