TY - JOUR
T1 - Cytotoxicity and mineralization potential of four calcium silicate-based cements on human gingiva-derived stem cells
AU - Lee, Donghee
AU - Park, Jun Beom
AU - Song, Dani
AU - Kim, Hye Min
AU - Kim, Sin Young
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - The aim of this study was to evaluate the cytotoxicity and mineralization potential of four calcium silicate-based cements on human gingiva-derived stem cells (GDSCs). The materials evaluated in the present study were ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), Endocem Zr (Maruchi), and RetroMTA (BioMTA). Experimental disks of 6 mm in diameter and 3 mm in height were produced and placed in a 100% humidified atmosphere for 48 h to set. We evaluated the cytotoxic effects of the cements using methyl-thiazoldiphenyl-tetrazolium (MTT) and live/dead staining assays. We used a scratch wound healing assay to evaluate cell migratory ability. Mineralization potential was determined with an Alizarin red S (ARS) staining assay. In the MTT assay, no significant differences were found among the ProRoot MTA, Biodentine, and control groups during the test period (p > 0.05). The Endocem Zr and RetroMTA groups showed relatively lower cell viability than the control group at day 7 (p < 0.05). In the wound healing assay, no significant differences were found among the ProRoot MTA, Biodentine, Endocem Zr, and control groups during the test period (p > 0.05). The RetroMTA group had slower cell migration compared to the control group at days 3 and 4 (p < 0.05). In the ARS assay, the ProRoot MTA, Biodentine, and RetroMTA groups exhibited a significant increase in the formation of mineralized nodules compared to the Endocem Zr and control groups on day 21 (p < 0.05). In conclusion, the four calcium silicate-based cements evaluated in the present study exhibited good biological properties on GDSCs. ProRoot MTA, Biodentine, and RetroMTA showed higher mineralization potential than the Endocem Zr and control groups.
AB - The aim of this study was to evaluate the cytotoxicity and mineralization potential of four calcium silicate-based cements on human gingiva-derived stem cells (GDSCs). The materials evaluated in the present study were ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), Endocem Zr (Maruchi), and RetroMTA (BioMTA). Experimental disks of 6 mm in diameter and 3 mm in height were produced and placed in a 100% humidified atmosphere for 48 h to set. We evaluated the cytotoxic effects of the cements using methyl-thiazoldiphenyl-tetrazolium (MTT) and live/dead staining assays. We used a scratch wound healing assay to evaluate cell migratory ability. Mineralization potential was determined with an Alizarin red S (ARS) staining assay. In the MTT assay, no significant differences were found among the ProRoot MTA, Biodentine, and control groups during the test period (p > 0.05). The Endocem Zr and RetroMTA groups showed relatively lower cell viability than the control group at day 7 (p < 0.05). In the wound healing assay, no significant differences were found among the ProRoot MTA, Biodentine, Endocem Zr, and control groups during the test period (p > 0.05). The RetroMTA group had slower cell migration compared to the control group at days 3 and 4 (p < 0.05). In the ARS assay, the ProRoot MTA, Biodentine, and RetroMTA groups exhibited a significant increase in the formation of mineralized nodules compared to the Endocem Zr and control groups on day 21 (p < 0.05). In conclusion, the four calcium silicate-based cements evaluated in the present study exhibited good biological properties on GDSCs. ProRoot MTA, Biodentine, and RetroMTA showed higher mineralization potential than the Endocem Zr and control groups.
KW - Calcium nodule formation
KW - Calcium silicate-based cements
KW - Cell migration assay
KW - Cell survival
UR - https://www.scopus.com/pages/publications/85083061775
U2 - 10.3390/coatings10030279
DO - 10.3390/coatings10030279
M3 - Article
AN - SCOPUS:85083061775
SN - 2079-6412
VL - 10
JO - Coatings
JF - Coatings
IS - 3
M1 - 279
ER -