Abstract
A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC/MS/MS) method for the determination of tiropramide in human plasma was developed. Tiropramide and internal standard, cisapride were extracted from human plasma by liquid-liquid extraction and analyzed on a Luna C8 column with the mobile phase of acetonitrile-ammonium formate (10mM, pH 4.5) (50:50, v/v). The analytes was detected using an electrospray ionization tandem mass spectrometry in the multiple-reaction-monitoring mode. The standard curve was linear (r=0.998) over the concentration range of 2.0-200ng/ml. The intra- and inter-assay coefficients of variation ranged from 2.8 to 7.8 and 6.7 to 8.9%, respectively. The recoveries of tiropramide ranged from 50.2 to 53.1%, with that of cisapride (internal standard) being 60.9±5.3%. The lower limit of quantification for tiropramide was 2.0ng/ml using 100μl plasma sample. This method was applied to the pharmacokinetic study of tiropramide in human.
| Original language | English |
|---|---|
| Pages (from-to) | 395-400 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 796 |
| Issue number | 2 |
| DOIs | |
| State | Published - 5 Nov 2003 |
Bibliographical note
Funding Information:This work was partially supported by the Project from the Center for Biological Modulators (CBM-02-B-8), Ministry of Science and Technology, Republic of Korea.
Keywords
- Tiropramide