Direct measurement of singlet oxygen by using a photomultiplier tube-based detection system

In Wook Kim; Jae Myung Park; Yoon Jin Roh; Ju Hee Kim; Myung Gyu Choi; Tayyaba Hasan

doi:10.1016/j.jphotobiol.2016.03.012

Direct measurement of singlet oxygen by using a photomultiplier tube-based detection system

In Wook Kim
, Jae Myung Park
, Yoon Jin Roh
, Ju Hee Kim
, Myung Gyu Choi
, Tayyaba Hasan

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The effective dosimetry for photodynamic therapy (PDT) can be specified by direct measurement of singlet oxygen (¹O₂) production. The purpose of this study was to investigate the feasibility of a newly developed photomultiplier tube (PMT)-based singlet oxygen detection (SOD) system. The lowest and highest ¹O₂ concentrations detectable by the PMT-SOD system were 15 nM and 10 μM, respectively. Dose-dependent quenching, by NaN_3, of the fluorogenic reaction was observed, which was negatively correlated with the ¹O₂ level measured by the PMT-SOD system. The lifetime of ¹O_2, as measured by the PMT-SOD system, was found to be lengthened when H₂O was replaced with deuterium oxide. ¹O₂ photon counts were significantly and dose-dependently correlated with intracellular fluorescence intensity after photosensitizer treatments. In vitro cell viability test and in vivo xenografted-tumor mass shrinkage showed a positive association between PDT-induced cytotoxicity and ¹O₂ production concomitantly measured by the PMT-SOD system. It was concluded that the PMT-SOD system is capable of measuring ¹O₂ production directly and accurately, demonstrating that this system can be useful in the determination of dosimetry for PDT.

Original language	English
Pages (from-to)	14-23
Number of pages	10
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	159
DOIs	https://doi.org/10.1016/j.jphotobiol.2016.03.012
State	Published - 1 Jun 2016

Bibliographical note

Publisher Copyright:
© 2016 Published by Elsevier B.V.

Keywords

Photodynamic therapy
Photosensitizer
Singlet oxygen
Validation

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10.1016/j.jphotobiol.2016.03.012

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title = "Direct measurement of singlet oxygen by using a photomultiplier tube-based detection system",

abstract = "The effective dosimetry for photodynamic therapy (PDT) can be specified by direct measurement of singlet oxygen (1O2) production. The purpose of this study was to investigate the feasibility of a newly developed photomultiplier tube (PMT)-based singlet oxygen detection (SOD) system. The lowest and highest 1O2 concentrations detectable by the PMT-SOD system were 15 nM and 10 μM, respectively. Dose-dependent quenching, by NaN3, of the fluorogenic reaction was observed, which was negatively correlated with the 1O2 level measured by the PMT-SOD system. The lifetime of 1O2, as measured by the PMT-SOD system, was found to be lengthened when H2O was replaced with deuterium oxide. 1O2 photon counts were significantly and dose-dependently correlated with intracellular fluorescence intensity after photosensitizer treatments. In vitro cell viability test and in vivo xenografted-tumor mass shrinkage showed a positive association between PDT-induced cytotoxicity and 1O2 production concomitantly measured by the PMT-SOD system. It was concluded that the PMT-SOD system is capable of measuring 1O2 production directly and accurately, demonstrating that this system can be useful in the determination of dosimetry for PDT.",

keywords = "Photodynamic therapy, Photosensitizer, Singlet oxygen, Validation",

author = "Kim, \{In Wook\} and Park, \{Jae Myung\} and Roh, \{Yoon Jin\} and Kim, \{Ju Hee\} and Choi, \{Myung Gyu\} and Tayyaba Hasan",

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year = "2016",

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doi = "10.1016/j.jphotobiol.2016.03.012",

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AU - Kim, In Wook

AU - Park, Jae Myung

AU - Roh, Yoon Jin

AU - Kim, Ju Hee

AU - Choi, Myung Gyu

AU - Hasan, Tayyaba

PY - 2016/6/1

Y1 - 2016/6/1

N2 - The effective dosimetry for photodynamic therapy (PDT) can be specified by direct measurement of singlet oxygen (1O2) production. The purpose of this study was to investigate the feasibility of a newly developed photomultiplier tube (PMT)-based singlet oxygen detection (SOD) system. The lowest and highest 1O2 concentrations detectable by the PMT-SOD system were 15 nM and 10 μM, respectively. Dose-dependent quenching, by NaN3, of the fluorogenic reaction was observed, which was negatively correlated with the 1O2 level measured by the PMT-SOD system. The lifetime of 1O2, as measured by the PMT-SOD system, was found to be lengthened when H2O was replaced with deuterium oxide. 1O2 photon counts were significantly and dose-dependently correlated with intracellular fluorescence intensity after photosensitizer treatments. In vitro cell viability test and in vivo xenografted-tumor mass shrinkage showed a positive association between PDT-induced cytotoxicity and 1O2 production concomitantly measured by the PMT-SOD system. It was concluded that the PMT-SOD system is capable of measuring 1O2 production directly and accurately, demonstrating that this system can be useful in the determination of dosimetry for PDT.

AB - The effective dosimetry for photodynamic therapy (PDT) can be specified by direct measurement of singlet oxygen (1O2) production. The purpose of this study was to investigate the feasibility of a newly developed photomultiplier tube (PMT)-based singlet oxygen detection (SOD) system. The lowest and highest 1O2 concentrations detectable by the PMT-SOD system were 15 nM and 10 μM, respectively. Dose-dependent quenching, by NaN3, of the fluorogenic reaction was observed, which was negatively correlated with the 1O2 level measured by the PMT-SOD system. The lifetime of 1O2, as measured by the PMT-SOD system, was found to be lengthened when H2O was replaced with deuterium oxide. 1O2 photon counts were significantly and dose-dependently correlated with intracellular fluorescence intensity after photosensitizer treatments. In vitro cell viability test and in vivo xenografted-tumor mass shrinkage showed a positive association between PDT-induced cytotoxicity and 1O2 production concomitantly measured by the PMT-SOD system. It was concluded that the PMT-SOD system is capable of measuring 1O2 production directly and accurately, demonstrating that this system can be useful in the determination of dosimetry for PDT.

KW - Photodynamic therapy

KW - Photosensitizer

KW - Singlet oxygen

KW - Validation

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M3 - Article

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SN - 1011-1344

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JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

ER -

Direct measurement of singlet oxygen by using a photomultiplier tube-based detection system

Abstract

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