Effect of nitric oxide and peroxynitrite on mucociliary transport function of experimental otitis media

Eun Ju Jeon; Yong Soo Park; Seung Kyun Lee; Sang Won Yeo; Shi Nae Park; Ki Hong Chang

doi:10.1016/j.otohns.2005.10.014

Effect of nitric oxide and peroxynitrite on mucociliary transport function of experimental otitis media

Eun Ju Jeon
, Yong Soo Park
, Seung Kyun Lee
, Sang Won Yeo
, Shi Nae Park
, Ki Hong Chang

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

OBJECTIVE: To determine the role of nitric oxide (NO) and peroxynitrite on mucociliary activity in experimental otitis media with effusion (OME). STUDY DESIGN AND SETTING: Twenty guinea pigs were divided into 1 control and 3 experimental groups; lipopolysaccharide (LPS), N^G-nitro-l-arginine methyl ester (l-NAME), and uric acid (UA) groups. Ten ears were used in each group. OME was induced by transtympanic injection of LPS in experimental groups. Twenty-four hours after the transtympanic injection, dye transfer time (DTT) was measured and the temporal bone was taken for histopathologic examination. Expression of peroxynitrite was determined by immunohistochemical stain for 3-nitrotyrosine (3-NT). RESULTS: Dye transfer time was significantly delayed in LPS group compared to control group; by contrast it was significantly reduced in l-NAME or UA treated groups (P < 0.01). Histopathologic examination showed reduced inflammation and mucosal thickening in the treatment groups when compared to LPS group. These findings, however, were not statistically significant. Immunoreactivity to 3-NT was intense in LPS group and decreased in the treatment groups (P < 0.05). CONCLUSIONS: It is suggested that LPS induced mucociliary dysfunction in the middle ear by NO and peroxynitrite- mediated pathways.

Original language	English
Pages (from-to)	126-131
Number of pages	6
Journal	Otolaryngology - Head and Neck Surgery
Volume	134
Issue number	1
DOIs	https://doi.org/10.1016/j.otohns.2005.10.014
State	Published - Jan 2006

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title = "Effect of nitric oxide and peroxynitrite on mucociliary transport function of experimental otitis media",

abstract = "OBJECTIVE: To determine the role of nitric oxide (NO) and peroxynitrite on mucociliary activity in experimental otitis media with effusion (OME). STUDY DESIGN AND SETTING: Twenty guinea pigs were divided into 1 control and 3 experimental groups; lipopolysaccharide (LPS), NG-nitro-l-arginine methyl ester (l-NAME), and uric acid (UA) groups. Ten ears were used in each group. OME was induced by transtympanic injection of LPS in experimental groups. Twenty-four hours after the transtympanic injection, dye transfer time (DTT) was measured and the temporal bone was taken for histopathologic examination. Expression of peroxynitrite was determined by immunohistochemical stain for 3-nitrotyrosine (3-NT). RESULTS: Dye transfer time was significantly delayed in LPS group compared to control group; by contrast it was significantly reduced in l-NAME or UA treated groups (P < 0.01). Histopathologic examination showed reduced inflammation and mucosal thickening in the treatment groups when compared to LPS group. These findings, however, were not statistically significant. Immunoreactivity to 3-NT was intense in LPS group and decreased in the treatment groups (P < 0.05). CONCLUSIONS: It is suggested that LPS induced mucociliary dysfunction in the middle ear by NO and peroxynitrite- mediated pathways.",

author = "Jeon, \{Eun Ju\} and Park, \{Yong Soo\} and Lee, \{Seung Kyun\} and Yeo, \{Sang Won\} and Park, \{Shi Nae\} and Chang, \{Ki Hong\}",

year = "2006",

month = jan,

doi = "10.1016/j.otohns.2005.10.014",

language = "English",

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T1 - Effect of nitric oxide and peroxynitrite on mucociliary transport function of experimental otitis media

AU - Jeon, Eun Ju

AU - Park, Yong Soo

AU - Lee, Seung Kyun

AU - Yeo, Sang Won

AU - Park, Shi Nae

AU - Chang, Ki Hong

PY - 2006/1

Y1 - 2006/1

N2 - OBJECTIVE: To determine the role of nitric oxide (NO) and peroxynitrite on mucociliary activity in experimental otitis media with effusion (OME). STUDY DESIGN AND SETTING: Twenty guinea pigs were divided into 1 control and 3 experimental groups; lipopolysaccharide (LPS), NG-nitro-l-arginine methyl ester (l-NAME), and uric acid (UA) groups. Ten ears were used in each group. OME was induced by transtympanic injection of LPS in experimental groups. Twenty-four hours after the transtympanic injection, dye transfer time (DTT) was measured and the temporal bone was taken for histopathologic examination. Expression of peroxynitrite was determined by immunohistochemical stain for 3-nitrotyrosine (3-NT). RESULTS: Dye transfer time was significantly delayed in LPS group compared to control group; by contrast it was significantly reduced in l-NAME or UA treated groups (P < 0.01). Histopathologic examination showed reduced inflammation and mucosal thickening in the treatment groups when compared to LPS group. These findings, however, were not statistically significant. Immunoreactivity to 3-NT was intense in LPS group and decreased in the treatment groups (P < 0.05). CONCLUSIONS: It is suggested that LPS induced mucociliary dysfunction in the middle ear by NO and peroxynitrite- mediated pathways.

AB - OBJECTIVE: To determine the role of nitric oxide (NO) and peroxynitrite on mucociliary activity in experimental otitis media with effusion (OME). STUDY DESIGN AND SETTING: Twenty guinea pigs were divided into 1 control and 3 experimental groups; lipopolysaccharide (LPS), NG-nitro-l-arginine methyl ester (l-NAME), and uric acid (UA) groups. Ten ears were used in each group. OME was induced by transtympanic injection of LPS in experimental groups. Twenty-four hours after the transtympanic injection, dye transfer time (DTT) was measured and the temporal bone was taken for histopathologic examination. Expression of peroxynitrite was determined by immunohistochemical stain for 3-nitrotyrosine (3-NT). RESULTS: Dye transfer time was significantly delayed in LPS group compared to control group; by contrast it was significantly reduced in l-NAME or UA treated groups (P < 0.01). Histopathologic examination showed reduced inflammation and mucosal thickening in the treatment groups when compared to LPS group. These findings, however, were not statistically significant. Immunoreactivity to 3-NT was intense in LPS group and decreased in the treatment groups (P < 0.05). CONCLUSIONS: It is suggested that LPS induced mucociliary dysfunction in the middle ear by NO and peroxynitrite- mediated pathways.

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DO - 10.1016/j.otohns.2005.10.014

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AN - SCOPUS:29944435865

SN - 0194-5998

VL - 134

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JO - Otolaryngology - Head and Neck Surgery

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IS - 1

ER -

Effect of nitric oxide and peroxynitrite on mucociliary transport function of experimental otitis media

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