Abstract
We published that bacterial heme was over-produced in a recombinant Corynebacterium glutamicum expressing 5-aminolevulinic acid synthase (hemA+) under control of a constitutive promoter (P180) and the heme-producing C. glutamicum had commercial potentials; as an iron feed additive for swine and as a preservative for lactic acid bacteria. To enhance the heme production, the hemA+ gene was expressed under controls of various promoters in the recombinant C. glutamicum. The hemA+ expression by PgapA (a constitutive glycolytic promoter of glyceraldehyde-3-phosphate dehydrogenase) led 75% increase of heme production while the expression by PH36 (a constitutive, very strong synthetic promoter) resulted in 50% decrease compared with the control (hemA+ expression by P180 constitutive promoter). The hemA+ expression by a late log-phase activating Psod (an oxidative-stress responding promoter of superoxide dismutase) led 50% greater heme production than the control. The hemA+ expression led by a heat-shock responding chaperone promoter (PdnaK) resulted in 121% increase of heme production at the optimized heat-shock conditions. The promoter strength and induction phase are discussed based on the results for the heme production at an industrial scale.
| Original language | English |
|---|---|
| Pages (from-to) | 337-342 |
| Number of pages | 6 |
| Journal | Microbiology and Biotechnology Letters |
| Volume | 47 |
| Issue number | 3 |
| DOIs | |
| State | Published - 28 Sep 2019 |
Bibliographical note
Funding Information:This work was supported by a funding from NRF of Korea (2016R1E1A1A01943552). The authors extend their appreciation to A3 Foresight Program (2016K2A9A2A10005545) for supporting research network.
Publisher Copyright:
© 2019, The Korean Society for Microbiology and Biotechnology
Keywords
- Heme
- P P
- P
- P
- P