Abstract
Platelet derived growth factor BB (PDGF BB) is a potent mitogenic, angiogenic and chemoattractant, and is one of the most abundant growth factors in platelet derived prod¬ucts. The goal of the present study was to examine the effects of PDGF BB on cellular morphology and cellular viability using 3D stem cell cultures. On day 1, spheroids formed well in silicon elastomer based concave microwells. The addition of 10 or 100 ng/ml PDGF BB did not affect the morphology of the cell spheroids. During longer periods of incubation, the cell spheroids maintained their shape without noticeable alterations. The majority of cells in the spheroids exhibited green fluorescence when analyzed using a live/dead assay, indicative of live cells. On day 1, the Cell Counting Kit 8 (CCK 8) assay values for PDGF BB at 0, 10 and 100 ng/ml were 0.241±0.003, 0.227±0.001 and 0.241±0.004, respectively; on day 3, the CCK 8 assay values for PDGF BB were 0.233±0.005, 0.278±0.001 and 0.194±0.003, respectively; and on day 7, they were 0.248±0.014, 0.293±0.031 and 0.346±0.034, respectively. The 100 ng/ml group showed significantly higher values compared with the control group on day 7. Together, the results of the present study showed that the addition of 10 and 100 ng/ml PDGF BB increased cellular viability, suggesting that PDGF BB may be usable in cell therapy.
| Original language | English |
|---|---|
| Article number | 59 |
| Pages (from-to) | 1-7 |
| Number of pages | 7 |
| Journal | Biomedical Reports |
| Volume | 13 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2020 |
Bibliographical note
Funding Information:This study was supported by a National Research Foundation of Korea grant funded by the Korean government (MSIT) (grant no. 2020R1A2C4001624) and by the Research Fund of Seoul St. Mary's Hospital, The Catholic University of Korea.
Publisher Copyright:
© 2020, Spandidos Publications. All rights reserved.
Keywords
- Bone marrow
- Cell survival
- Cellular spheroids
- Platelet derived growth factor
- Stem cells