Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology

Ji Young Hong; Byunghak Kang; Ahyoun Kim; Seoyoun Hwang; Sunwha Lee; Jonghyen Kim; Hyun Young Lee; Sang Hyeon Kang; Doo Sung Cheon

doi:10.1128/JCM.01790-09

Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology

Ji Young Hong, Byunghak Kang, Ahyoun Kim, Seoyoun Hwang, Sunwha Lee, Jonghyen Kim, Hyun Young Lee, Sang Hyeon Kang, Doo Sung Cheon

Department of Pediatrics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

Original language	English
Pages (from-to)	615-616
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	48
Issue number	2
DOIs	https://doi.org/10.1128/JCM.01790-09
State	Published - Feb 2010

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title = "Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology",

abstract = "To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.",

author = "Hong, \{Ji Young\} and Byunghak Kang and Ahyoun Kim and Seoyoun Hwang and Sunwha Lee and Jonghyen Kim and Lee, \{Hyun Young\} and Kang, \{Sang Hyeon\} and Cheon, \{Doo Sung\}",

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AU - Hong, Ji Young

AU - Kang, Byunghak

AU - Kim, Ahyoun

AU - Hwang, Seoyoun

AU - Lee, Sunwha

AU - Kim, Jonghyen

AU - Lee, Hyun Young

AU - Kang, Sang Hyeon

AU - Cheon, Doo Sung

PY - 2010/2

Y1 - 2010/2

N2 - To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

AB - To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

UR - https://www.scopus.com/pages/publications/75649094550

U2 - 10.1128/JCM.01790-09

DO - 10.1128/JCM.01790-09

M3 - Article

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AN - SCOPUS:75649094550

SN - 0095-1137

VL - 48

SP - 615

EP - 616

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -

Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology

Abstract

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