Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes

Kun Na; Seong Jun Choi; Sungwon Kim; Bo Kyung Sun; Dae Gyun Woo; Hyung Min Chung; Keun Hong Park

doi:10.1007/s10529-007-9420-8

Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes

Kun Na
, Seong Jun Choi
, Sungwon Kim
, Bo Kyung Sun
, Dae Gyun Woo
, Hyung Min Chung
, Keun Hong Park

CHA University
Korea Institute of Science and Technology
Chabiotech Co., Ltd.

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The effects of inducible materials (dexamethasone and ascorbate) on chondrogenic differentiation of rabbit chondrocytes have been examined. A hydrogel construct containing dexamethasone and ascorbate up-regulated gene expression of the cartilage matrix component of collagen to give three times the collagen content per construct at day 56 as compared to controls. Alcian Blue and Safranin-O staining revealed that these constructs also had formed more hyaline cartilage than other hydrogel constructs.

Original language	English
Pages (from-to)	1453-1457
Number of pages	5
Journal	Biotechnology Letters
Volume	29
Issue number	10
DOIs	https://doi.org/10.1007/s10529-007-9420-8
State	Published - Oct 2007

Bibliographical note

Funding Information:
Acknowledgements This work was supported by a Korea Research Foundation Grant (KRF2005-D00961).

Keywords

Ascorbate
Cartilage
Chondrocytes
Dexamethasone
Hydrogel

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10.1007/s10529-007-9420-8

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title = "Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes",

abstract = "The effects of inducible materials (dexamethasone and ascorbate) on chondrogenic differentiation of rabbit chondrocytes have been examined. A hydrogel construct containing dexamethasone and ascorbate up-regulated gene expression of the cartilage matrix component of collagen to give three times the collagen content per construct at day 56 as compared to controls. Alcian Blue and Safranin-O staining revealed that these constructs also had formed more hyaline cartilage than other hydrogel constructs.",

keywords = "Ascorbate, Cartilage, Chondrocytes, Dexamethasone, Hydrogel",

author = "Kun Na and Choi, \{Seong Jun\} and Sungwon Kim and Sun, \{Bo Kyung\} and Woo, \{Dae Gyun\} and Chung, \{Hyung Min\} and Park, \{Keun Hong\}",

note = "Funding Information: Acknowledgements This work was supported by a Korea Research Foundation Grant (KRF2005-D00961).",

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T1 - Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes

AU - Na, Kun

AU - Choi, Seong Jun

AU - Kim, Sungwon

AU - Sun, Bo Kyung

AU - Woo, Dae Gyun

AU - Chung, Hyung Min

AU - Park, Keun Hong

N1 - Funding Information: Acknowledgements This work was supported by a Korea Research Foundation Grant (KRF2005-D00961).

PY - 2007/10

Y1 - 2007/10

N2 - The effects of inducible materials (dexamethasone and ascorbate) on chondrogenic differentiation of rabbit chondrocytes have been examined. A hydrogel construct containing dexamethasone and ascorbate up-regulated gene expression of the cartilage matrix component of collagen to give three times the collagen content per construct at day 56 as compared to controls. Alcian Blue and Safranin-O staining revealed that these constructs also had formed more hyaline cartilage than other hydrogel constructs.

AB - The effects of inducible materials (dexamethasone and ascorbate) on chondrogenic differentiation of rabbit chondrocytes have been examined. A hydrogel construct containing dexamethasone and ascorbate up-regulated gene expression of the cartilage matrix component of collagen to give three times the collagen content per construct at day 56 as compared to controls. Alcian Blue and Safranin-O staining revealed that these constructs also had formed more hyaline cartilage than other hydrogel constructs.

KW - Ascorbate

KW - Cartilage

KW - Chondrocytes

KW - Dexamethasone

KW - Hydrogel

UR - https://www.scopus.com/pages/publications/34548131694

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DO - 10.1007/s10529-007-9420-8

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SP - 1453

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 10

ER -

Enhancement of cell proliferation and differentiation by combination of ascorbate and dexamethasone in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes

Abstract

Bibliographical note

Keywords

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