Enzymatic synthesis of novel phloretin glucosides

Ramesh Prasad Pandey; Tai Feng Li; Eun Hee Kim; Tokutaro Yamaguchi; Yong Il Park; Joong Su Kim; Jae Kyung Sohng

doi:10.1128/AEM.00409-13

Enzymatic synthesis of novel phloretin glucosides

Ramesh Prasad Pandey
, Tai Feng Li
, Eun Hee Kim
, Tokutaro Yamaguchi
, Yong Il Park
, Joong Su Kim
, Jae Kyung Sohng

Department of Medical and Biological Sciences

Sun Moon University
Korea Basic Science Institute
Korea Research Institute of Bioscience and Biotechnology

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli.

Original language	English
Pages (from-to)	3516-3521
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	11
DOIs	https://doi.org/10.1128/AEM.00409-13
State	Published - Jun 2013

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title = "Enzymatic synthesis of novel phloretin glucosides",

abstract = "A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli.",

author = "Pandey, \{Ramesh Prasad\} and Li, \{Tai Feng\} and Kim, \{Eun Hee\} and Tokutaro Yamaguchi and Park, \{Yong Il\} and Kim, \{Joong Su\} and Sohng, \{Jae Kyung\}",

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AU - Pandey, Ramesh Prasad

AU - Li, Tai Feng

AU - Kim, Eun Hee

AU - Yamaguchi, Tokutaro

AU - Park, Yong Il

AU - Kim, Joong Su

AU - Sohng, Jae Kyung

PY - 2013/6

Y1 - 2013/6

N2 - A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli.

AB - A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli.

UR - https://www.scopus.com/pages/publications/84877154435

U2 - 10.1128/AEM.00409-13

DO - 10.1128/AEM.00409-13

M3 - Article

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AN - SCOPUS:84877154435

SN - 0099-2240

VL - 79

SP - 3516

EP - 3521

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 11

ER -

Enzymatic synthesis of novel phloretin glucosides

Abstract

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