Evidence for increased NADPH-diaphorase-positive neurons in the central auditory system of the aged rat

Youngbuhm Huh, Dong Choon Park, Seung Geun Yeo, Chang Cha Il

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8 Scopus citations

Abstract

Conclusions: The age-related increase in the production of nitric oxide (NO) suggests that this increase was related to neuron aging. Additional studies may provide information regarding aging-related changes in the central auditory system. Objectives: Although NO has been associated with aging, it is unclear whether specific areas of the central auditory system are involved. We therefore assayed aging-related changes in NADPH-diaphorase (NADPH-d), a selective histochemical marker for NO, in the neurons of the central auditory system and other brain regions. Materials and methods: The numbers of NADPH-d-stained neurons and the area and staining density of cell bodies were examined in aged (24 months old) and younger (4 months old) Wistar rats. Results: The number of NADPH-d-positive neurons in the inferior colliculus was significantly increased in aged rats (p<0.05), whereas the area of NADPH-d-positive neurons in all areas did not differ significantly between aged and younger rats (p>0.05). The staining densities of NADPH-d-positive neurons in the inferior colliculus, the auditory cortex, and the visual cortex were significantly greater in aged compared with younger rats (p<0.05).

Original languageEnglish
Pages (from-to)648-653
Number of pages6
JournalActa Oto-Laryngologica
Volume128
Issue number6
DOIs
StatePublished - 2008

Bibliographical note

Funding Information:
This research was supported by the Kyung Hee University Research Fund in 2006 (KHU-2006-064).

Keywords

  • Aging
  • Auditory cortex
  • Cochlear nucleus
  • Inferior colliculus
  • Nicotinamide adenine dinucleotide phosphate

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