Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs

Sergiy Velychko; Kenjiro Adachi; Kee Pyo Kim; Yanlin Hou; Caitlin M. MacCarthy; Guangming Wu; Hans R. Schöler

doi:10.1016/j.stem.2019.10.002

Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs

Sergiy Velychko
, Kenjiro Adachi
, Kee Pyo Kim
, Yanlin Hou
, Caitlin M. MacCarthy
, Guangming Wu
, Hans R. Schöler

Department of Biomedicine & Health Science

Max Planck Institute for Molecular Biomedicine
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
University of Münster

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

Velychko et al. showed that Oct4 overexpression during reprogramming causes epigenetic changes deteriorating the quality of iPSCs. SKM reprogramming generates iPSCs with equivalent developmental potential to ESCs, as determined by their ability to generate all-iPSC mice through tetraploid embryo complementation.

Original language	English
Pages (from-to)	737-753.e4
Journal	Cell Stem Cell
Volume	25
Issue number	6
DOIs	https://doi.org/10.1016/j.stem.2019.10.002
State	Published - 5 Dec 2019

Bibliographical note

Publisher Copyright:
© 2019 The Authors

Keywords

Klf4
Myc
Oct4
Sox2
Yamanaka cocktail
developmental potential
induced pluripotent stem cells
reprogramming
retroviral silencing
seesaw model of pluripotency
tetraploid complementation
transcription factor

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10.1016/j.stem.2019.10.002

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title = "Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs",

abstract = "Velychko et al. showed that Oct4 overexpression during reprogramming causes epigenetic changes deteriorating the quality of iPSCs. SKM reprogramming generates iPSCs with equivalent developmental potential to ESCs, as determined by their ability to generate all-iPSC mice through tetraploid embryo complementation.",

keywords = "Klf4, Myc, Oct4, Sox2, Yamanaka cocktail, developmental potential, induced pluripotent stem cells, reprogramming, retroviral silencing, seesaw model of pluripotency, tetraploid complementation, transcription factor",

author = "Sergiy Velychko and Kenjiro Adachi and Kim, \{Kee Pyo\} and Yanlin Hou and MacCarthy, \{Caitlin M.\} and Guangming Wu and Sch{\"o}ler, \{Hans R.\}",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors",

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month = dec,

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AU - Velychko, Sergiy

AU - Adachi, Kenjiro

AU - Kim, Kee Pyo

AU - Hou, Yanlin

AU - MacCarthy, Caitlin M.

AU - Wu, Guangming

AU - Schöler, Hans R.

PY - 2019/12/5

Y1 - 2019/12/5

N2 - Velychko et al. showed that Oct4 overexpression during reprogramming causes epigenetic changes deteriorating the quality of iPSCs. SKM reprogramming generates iPSCs with equivalent developmental potential to ESCs, as determined by their ability to generate all-iPSC mice through tetraploid embryo complementation.

AB - Velychko et al. showed that Oct4 overexpression during reprogramming causes epigenetic changes deteriorating the quality of iPSCs. SKM reprogramming generates iPSCs with equivalent developmental potential to ESCs, as determined by their ability to generate all-iPSC mice through tetraploid embryo complementation.

KW - Klf4

KW - Myc

KW - Oct4

KW - Sox2

KW - Yamanaka cocktail

KW - developmental potential

KW - induced pluripotent stem cells

KW - reprogramming

KW - retroviral silencing

KW - seesaw model of pluripotency

KW - tetraploid complementation

KW - transcription factor

UR - https://www.scopus.com/pages/publications/85075584082

U2 - 10.1016/j.stem.2019.10.002

DO - 10.1016/j.stem.2019.10.002

M3 - Article

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AN - SCOPUS:85075584082

SN - 1934-5909

VL - 25

SP - 737-753.e4

JO - Cell Stem Cell

JF - Cell Stem Cell

IS - 6

ER -

Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs

Abstract

Bibliographical note

Keywords

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