Exon/intron organization and transcription units of the human acyl-CoA synthetase 4 gene

Hiroyuki Minekura; Man Jong Kang; Yousuke Inagaki; Yong Yeon Cho; Hiroyuki Suzuki; Takahiro Fujino; Tokuo T. Yamamoto

doi:10.1006/bbrc.2001.5357

Exon/intron organization and transcription units of the human acyl-CoA synthetase 4 gene

Hiroyuki Minekura
, Man Jong Kang
, Yousuke Inagaki
, Yong Yeon Cho
, Hiroyuki Suzuki
, Takahiro Fujino
, Tokuo T. Yamamoto

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring isozyme of ACS family predominantly expressed in steroidogenic tissues. Isolation and characterization of genomic clones encoding human ACS4 revealed that the genomic organization of the gene. The human ACS4 gene spans approximately 90 kb and consists of 16 exons. Sequence inspection of the 5′-flanking region revealed potential DNA elements including GATAs, p300, AP-4, SRY, CREB and MyoD. A minimal promoter region required for the expression of ACS4 in HeLa S3 cells was determined. The human ACS4 gene was mapped between the STS markers, WI-17685 and CHLC.GATA81B07 on Xq22-23 region.

Original language	English
Pages (from-to)	80-86
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	286
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.2001.5357
State	Published - 2001

Bibliographical note

Funding Information:
This work was supported by Japan Society for Promotion of Science Grant RFTF97L00803.

Keywords

Acyl-CoA synthetase
Arachidonate
Fatty acid metabolism
Genomic organization

Access to Document

10.1006/bbrc.2001.5357

Cite this

@article{47ebbc34bff546b08a3c2b7c83d17c4e,

title = "Exon/intron organization and transcription units of the human acyl-CoA synthetase 4 gene",

abstract = "Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring isozyme of ACS family predominantly expressed in steroidogenic tissues. Isolation and characterization of genomic clones encoding human ACS4 revealed that the genomic organization of the gene. The human ACS4 gene spans approximately 90 kb and consists of 16 exons. Sequence inspection of the 5′-flanking region revealed potential DNA elements including GATAs, p300, AP-4, SRY, CREB and MyoD. A minimal promoter region required for the expression of ACS4 in HeLa S3 cells was determined. The human ACS4 gene was mapped between the STS markers, WI-17685 and CHLC.GATA81B07 on Xq22-23 region.",

keywords = "Acyl-CoA synthetase, Arachidonate, Fatty acid metabolism, Genomic organization",

author = "Hiroyuki Minekura and Kang, \{Man Jong\} and Yousuke Inagaki and Cho, \{Yong Yeon\} and Hiroyuki Suzuki and Takahiro Fujino and Yamamoto, \{Tokuo T.\}",

note = "Funding Information: This work was supported by Japan Society for Promotion of Science Grant RFTF97L00803.",

year = "2001",

doi = "10.1006/bbrc.2001.5357",

language = "English",

volume = "286",

pages = "80--86",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Exon/intron organization and transcription units of the human acyl-CoA synthetase 4 gene

AU - Minekura, Hiroyuki

AU - Kang, Man Jong

AU - Inagaki, Yousuke

AU - Cho, Yong Yeon

AU - Suzuki, Hiroyuki

AU - Fujino, Takahiro

AU - Yamamoto, Tokuo T.

N1 - Funding Information: This work was supported by Japan Society for Promotion of Science Grant RFTF97L00803.

PY - 2001

Y1 - 2001

N2 - Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring isozyme of ACS family predominantly expressed in steroidogenic tissues. Isolation and characterization of genomic clones encoding human ACS4 revealed that the genomic organization of the gene. The human ACS4 gene spans approximately 90 kb and consists of 16 exons. Sequence inspection of the 5′-flanking region revealed potential DNA elements including GATAs, p300, AP-4, SRY, CREB and MyoD. A minimal promoter region required for the expression of ACS4 in HeLa S3 cells was determined. The human ACS4 gene was mapped between the STS markers, WI-17685 and CHLC.GATA81B07 on Xq22-23 region.

AB - Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring isozyme of ACS family predominantly expressed in steroidogenic tissues. Isolation and characterization of genomic clones encoding human ACS4 revealed that the genomic organization of the gene. The human ACS4 gene spans approximately 90 kb and consists of 16 exons. Sequence inspection of the 5′-flanking region revealed potential DNA elements including GATAs, p300, AP-4, SRY, CREB and MyoD. A minimal promoter region required for the expression of ACS4 in HeLa S3 cells was determined. The human ACS4 gene was mapped between the STS markers, WI-17685 and CHLC.GATA81B07 on Xq22-23 region.

KW - Acyl-CoA synthetase

KW - Arachidonate

KW - Fatty acid metabolism

KW - Genomic organization

UR - https://www.scopus.com/pages/publications/0034804630

U2 - 10.1006/bbrc.2001.5357

DO - 10.1006/bbrc.2001.5357

M3 - Article

C2 - 11485311

AN - SCOPUS:0034804630

SN - 0006-291X

VL - 286

SP - 80

EP - 86

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Exon/intron organization and transcription units of the human acyl-CoA synthetase 4 gene

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this