Expression of interferon inducible genes following Hantaan virus infection as a mechanism of resistance in A549 cells

Jae Hwan Nam, Kyung A. Hwang, Cheong Hee Yu, Tae Hoon Kang, Jae Young Shin, Woo Young Choi, In Beom Kim, Young Ran Joo, Hae Wol Cho, Keun Yong Park

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Hantaan virus (HTN) is a causative agent of hemorrhagic fever with renal syndrome (HFRS). Little is known of its pathogenesis or the molecular mechanisms underlying resistance to HTN infection. In the present study, DNA microarray technology was used to monitor changes in mRNA levels after HTN infection, to elucidate resistance mechanisms to viral infection by understanding virus-host interactions. We found that several interferon (IFN)-inducible genes were up-regulated in host cells infected with HTN. According to previous available data, IFNs have been reported to be inhibitory, but their mode of action has not been yet clear. In this study, the 2′,5′-oligoadenylated synthetase (OAS) and Mx1 genes, not a double-stranded RNA-dependent protein kinase R (PKR), of the IFN response pathways are associated with antiviral activity during HTN infection. Furthermore, A549 cells treated with IFN-α were protected against HTN infection. Taken together, these results confirmed that IFN plays a role in cellular defenses against HTN infection at an early stage of the infection and revealed the resistance mechanism for HTN infection.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalVirus Genes
Volume26
Issue number1
DOIs
StatePublished - 2003

Bibliographical note

Funding Information:
We thank Drs. Mingdong Zhang and Rajen Koshy for carefully reading the article in manuscript and for providing helpful suggestions. This study was supported by a grant from the Korean National Institute of Health.

Keywords

  • 2′,5′-oligoadenylated synthetase (OAS)
  • cDNA microarray
  • Hantaan virus
  • Interferon
  • M1

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