Abstract
High levels of glutamate can be toxic to retinal ganglion cells (RGCs). This study investigated the relationship between the N-methyl-d-aspartate receptor 1 (NR) and RGC death in a rat model of chronic ocular hypertension (COHT). COHT was induced in one eye of each rat by episcleral vein cauterization. Retinal protein expression was evaluated at 1, 3, 5 and 9 weeks after cauterization. Quantitative real-time polymerase chain reaction and Western blot analysis showed that NR1 expression was significantly increased in cauterized retinae. NR1 immunoreactivity was observed in the inner nuclear layer (INL) and ganglion cell layer (GCL) in the retina of rats with COHT. RGC density was evaluated after retrograde labeling with fluoro-gold (FG) and 4-di-10-ASP (DiA). A significant decrease in RGC density was observed in ocular hypertensive eyes, and NR1 expression in the GCL suggested an important role of NR1 in the death of RGCs. Memantine (10 mg/kg), an N-methyl-d-aspartate receptor antagonist, was administered orally once daily for up to 5 weeks, while rats in the control group received vehicle phosphate-buffered saline only. Treatment with memantine resulted in a significant reduction in RGC loss and NR1 expression in the eyes of rats COHT. These findings suggest that excessive expression of NR1 is involved in RGC death in glaucoma.
| Original language | English |
|---|---|
| Pages (from-to) | 908-916 |
| Number of pages | 9 |
| Journal | Neuroscience |
| Volume | 149 |
| Issue number | 4 |
| DOIs | |
| State | Published - 23 Nov 2007 |
Bibliographical note
Funding Information:The authors would like to thank Rossana Romani (Allergan, Australia) for reading and commenting on the manuscript and verifying English usage. This study was supported in part by a grant from the Catholic Medical Center Research Foundation and a nonrestricted grant for Scientific/Educational Activity from Allergan Korea Ltd., Seoul, Korea.
Keywords
- NMDA receptor
- chronic ocular hypertension
- glutamate
- retinal ganglion cell