Expression profiling of microRNAs in lipopolysaccharide-induced acute lung injury after hypothermia treatment

Woonjeong Lee; Insoo Kim; Soyoung Shin; Kicheol Park; Keumjin Yang; Jung Woo Eun; Haejoung Sul; Sikyoung Jeong

doi:10.1007/s13273-016-0029-7

Expression profiling of microRNAs in lipopolysaccharide-induced acute lung injury after hypothermia treatment

Woonjeong Lee, Insoo Kim, Soyoung Shin, Kicheol Park, Keumjin Yang, Jung Woo Eun, Haejoung Sul, Sikyoung Jeong

Department of Emergency Medicine

The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We investigated the expression profiles of miRNAs in acute lung injury (ALI) rats after hypothermia treatment. ALI rats were induced with lipopolysaccharide (LPS) and maintained with hypothermia (HT) or normothermia (NT) for 6 hours. HT attenuated inflammatory cell infiltration in the lung and improved biochemical indicators of multi-organ dysfunction. Nineteen miRNAs were significantly differentially expressed in the HT group compared with the NT group. miR-142, miR-98, miR-541, miR-503, miR-653, miR- 223, miR-323 and miR-196b exhibited opposite patterns of expression between the two groups. These dysregulated miRNAs were mainly involved in the immune and inflammatory response on functional annotation analyses. This study shows that HT has lung protective effects and influences expression profiles of miRNAs in ALI. And dysregulated miRNAs after HT modulate the immune and inflammation in ALI. These results suggest that dysregulated miRNAs play a role in the mechanism of the lung protective effects of HT in ALI.

Original language	English
Pages (from-to)	243-253
Number of pages	11
Journal	Molecular and Cellular Toxicology
Volume	12
Issue number	3
DOIs	https://doi.org/10.1007/s13273-016-0029-7
State	Published - 1 Sep 2016

Bibliographical note

Publisher Copyright:
© 2016, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht.

Keywords

Acute lung injury
Hypothermia
MicroRNA

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s13273-016-0029-7

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title = "Expression profiling of microRNAs in lipopolysaccharide-induced acute lung injury after hypothermia treatment",

abstract = "We investigated the expression profiles of miRNAs in acute lung injury (ALI) rats after hypothermia treatment. ALI rats were induced with lipopolysaccharide (LPS) and maintained with hypothermia (HT) or normothermia (NT) for 6 hours. HT attenuated inflammatory cell infiltration in the lung and improved biochemical indicators of multi-organ dysfunction. Nineteen miRNAs were significantly differentially expressed in the HT group compared with the NT group. miR-142, miR-98, miR-541, miR-503, miR-653, miR- 223, miR-323 and miR-196b exhibited opposite patterns of expression between the two groups. These dysregulated miRNAs were mainly involved in the immune and inflammatory response on functional annotation analyses. This study shows that HT has lung protective effects and influences expression profiles of miRNAs in ALI. And dysregulated miRNAs after HT modulate the immune and inflammation in ALI. These results suggest that dysregulated miRNAs play a role in the mechanism of the lung protective effects of HT in ALI.",

keywords = "Acute lung injury, Hypothermia, MicroRNA",

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AU - Lee, Woonjeong

AU - Kim, Insoo

AU - Shin, Soyoung

AU - Park, Kicheol

AU - Yang, Keumjin

AU - Eun, Jung Woo

AU - Sul, Haejoung

AU - Jeong, Sikyoung

PY - 2016/9/1

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N2 - We investigated the expression profiles of miRNAs in acute lung injury (ALI) rats after hypothermia treatment. ALI rats were induced with lipopolysaccharide (LPS) and maintained with hypothermia (HT) or normothermia (NT) for 6 hours. HT attenuated inflammatory cell infiltration in the lung and improved biochemical indicators of multi-organ dysfunction. Nineteen miRNAs were significantly differentially expressed in the HT group compared with the NT group. miR-142, miR-98, miR-541, miR-503, miR-653, miR- 223, miR-323 and miR-196b exhibited opposite patterns of expression between the two groups. These dysregulated miRNAs were mainly involved in the immune and inflammatory response on functional annotation analyses. This study shows that HT has lung protective effects and influences expression profiles of miRNAs in ALI. And dysregulated miRNAs after HT modulate the immune and inflammation in ALI. These results suggest that dysregulated miRNAs play a role in the mechanism of the lung protective effects of HT in ALI.

AB - We investigated the expression profiles of miRNAs in acute lung injury (ALI) rats after hypothermia treatment. ALI rats were induced with lipopolysaccharide (LPS) and maintained with hypothermia (HT) or normothermia (NT) for 6 hours. HT attenuated inflammatory cell infiltration in the lung and improved biochemical indicators of multi-organ dysfunction. Nineteen miRNAs were significantly differentially expressed in the HT group compared with the NT group. miR-142, miR-98, miR-541, miR-503, miR-653, miR- 223, miR-323 and miR-196b exhibited opposite patterns of expression between the two groups. These dysregulated miRNAs were mainly involved in the immune and inflammatory response on functional annotation analyses. This study shows that HT has lung protective effects and influences expression profiles of miRNAs in ALI. And dysregulated miRNAs after HT modulate the immune and inflammation in ALI. These results suggest that dysregulated miRNAs play a role in the mechanism of the lung protective effects of HT in ALI.

KW - Acute lung injury

KW - Hypothermia

KW - MicroRNA

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Expression profiling of microRNAs in lipopolysaccharide-induced acute lung injury after hypothermia treatment

Abstract

Bibliographical note

Keywords

UN SDGs

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