Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling

Xiao Hui Tan; Sheng Ji Gu; Wen Jie Tian; Wen Peng Song; Yang Yang Gu; Yi Ming Yuan; Xue Song Li; Zhong Cheng Xin; Sae Woong Kim; Rui Li Guan; Woong Jin Bae

doi:10.1007/s11033-022-07316-1

Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling

Xiao Hui Tan, Sheng Ji Gu, Wen Jie Tian, Wen Peng Song, Yang Yang Gu, Yi Ming Yuan, Xue Song Li, Zhong Cheng Xin, Sae Woong Kim, Rui Li Guan, Woong Jin Bae

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5 Scopus citations

Abstract

Background: Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated. Methods: EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs. Results: A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia. Conclusions: Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility.

Original language	English
Pages (from-to)	4673-4681
Number of pages	9
Journal	Molecular Biology Reports
Volume	49
Issue number	6
DOIs	https://doi.org/10.1007/s11033-022-07316-1
State	Published - Jun 2022

Bibliographical note

Funding Information:
The authors would like to thank the donor of human Sertoli cells, who made this study possible. We also appreciate the Human Protein Atlas and the PRIDE, GEO, GO, KEGG databases for providing their platforms as well as contributors for uploading their meaningful datasets.

Funding Information:
The work was supported by the Horizontal Projects and the National Natural Science Foundation of China (Grant No. 81401194).

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.

Keywords

Extracellular vesicles
microRNAs
Proteomics
Sertoli cells
Spermiogenesis

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10.1007/s11033-022-07316-1

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title = "Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling",

abstract = "Background: Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated. Methods: EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs. Results: A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia. Conclusions: Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility.",

keywords = "Extracellular vesicles, microRNAs, Proteomics, Sertoli cells, Spermiogenesis",

author = "Tan, {Xiao Hui} and Gu, {Sheng Ji} and Tian, {Wen Jie} and Song, {Wen Peng} and Gu, {Yang Yang} and Yuan, {Yi Ming} and Li, {Xue Song} and Xin, {Zhong Cheng} and Kim, {Sae Woong} and Guan, {Rui Li} and Bae, {Woong Jin}",

note = "Funding Information: The authors would like to thank the donor of human Sertoli cells, who made this study possible. We also appreciate the Human Protein Atlas and the PRIDE, GEO, GO, KEGG databases for providing their platforms as well as contributors for uploading their meaningful datasets. Funding Information: The work was supported by the Horizontal Projects and the National Natural Science Foundation of China (Grant No. 81401194). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature B.V.",

year = "2022",

month = jun,

doi = "10.1007/s11033-022-07316-1",

language = "English",

volume = "49",

pages = "4673--4681",

journal = "Molecular Biology Reports",

issn = "0301-4851",

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T1 - Extracellular vesicles derived from human Sertoli cells

T2 - characterizations, proteomic analysis, and miRNA profiling

AU - Tan, Xiao Hui

AU - Gu, Sheng Ji

AU - Tian, Wen Jie

AU - Song, Wen Peng

AU - Gu, Yang Yang

AU - Yuan, Yi Ming

AU - Li, Xue Song

AU - Xin, Zhong Cheng

AU - Kim, Sae Woong

AU - Guan, Rui Li

AU - Bae, Woong Jin

N1 - Funding Information: The authors would like to thank the donor of human Sertoli cells, who made this study possible. We also appreciate the Human Protein Atlas and the PRIDE, GEO, GO, KEGG databases for providing their platforms as well as contributors for uploading their meaningful datasets. Funding Information: The work was supported by the Horizontal Projects and the National Natural Science Foundation of China (Grant No. 81401194). Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer Nature B.V.

PY - 2022/6

Y1 - 2022/6

N2 - Background: Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated. Methods: EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs. Results: A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia. Conclusions: Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility.

AB - Background: Extracellular vesicles (EVs) contain thousands of proteins and nucleic acids, playing an important role in cell–cell communications. Sertoli cells have been essential in the testis as a “nurse cell”. However, EVs derived from human Sertoli cells (HSerCs) have not been well investigated. Methods: EVs were isolated from HSerCs via ultracentrifugation and characterized by transmission electron microscopy, tunable resistive pulse sensing, and Western blotting. The cargo carried by HSerCs-EVs was measured via liquid chromatography-mass spectrometry and GeneChip miRNA Arrays. Bioinformatic analysis was performed to reveal potential functions of HSerCs-EVs. Results: A total of 860 proteins with no less than 2 unique peptides and 88 microRNAs with high signal values were identified in HSerCs-EVs. Biological processes related to molecular binding, enzyme activity, and regulation of cell cycle were significantly enriched. Specifically, many proteins in HSerCs-EVs were associated with spermatogenesis and regulation of immune system, including Septins, Large proline-rich protein BAG6, Clusterin, and Galectin-1. Moreover, abundant microRNAs within HSerCs-EVs (miR-638, miR-149-3p, miR-1246, etc.) had a possible impact on male reproductive disorders such as asthenozoospermia and oligozoospermia. Conclusions: Our study has shown that HSerCs-EVs contain diverse components such as proteins and microRNAs. Further research is required to evaluate HSerCs-EVs in spermatogenesis, which are underutilized but highly potent resources with particular promise for male infertility.

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KW - microRNAs

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KW - Spermiogenesis

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Extracellular vesicles derived from human Sertoli cells: characterizations, proteomic analysis, and miRNA profiling

Abstract

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Keywords

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