Fabrication of dihydroxyflavone-conjugated hyaluronic acid nanogels for targeted antitumoral effect

Yu Ri Choi; Hyun Jong Kim; Guk Young Ahn; Min Jeong Lee; Ju Ri Park; Dae Ryong Jun; Tae Kyoung Ryu; Joo Woong Park; Eunji Shin; Sung Wook Choi

doi:10.1016/j.colsurfb.2018.08.003

Fabrication of dihydroxyflavone-conjugated hyaluronic acid nanogels for targeted antitumoral effect

Yu Ri Choi, Hyun Jong Kim, Guk Young Ahn, Min Jeong Lee, Ju Ri Park, Dae Ryong Jun, Tae Kyoung Ryu, Joo Woong Park, Eunji Shin, Sung Wook Choi

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels.

Original language	English
Pages (from-to)	690-697
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	171
DOIs	https://doi.org/10.1016/j.colsurfb.2018.08.003
State	Published - 1 Nov 2018

Bibliographical note

Funding Information:
This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning ( NRF-2015R1A4A1042350 and 2017R1A2B4008093 ), a research fund of Biostream Technologies, R&D program of KITECH, the Research Fund, 2018 of The Catholic University of Korea, and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry Health & Welfare, Republic of Korea ( HI17C0886 ).

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

Dihydroxyflavone
Hyaluronic acid
Nanogel
Tumor therapy

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10.1016/j.colsurfb.2018.08.003

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@article{28c8975b32b94e40a75c1620b06c1106,

title = "Fabrication of dihydroxyflavone-conjugated hyaluronic acid nanogels for targeted antitumoral effect",

abstract = "We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels.",

keywords = "Dihydroxyflavone, Hyaluronic acid, Nanogel, Tumor therapy",

author = "Choi, {Yu Ri} and Kim, {Hyun Jong} and Ahn, {Guk Young} and Lee, {Min Jeong} and Park, {Ju Ri} and Jun, {Dae Ryong} and Ryu, {Tae Kyoung} and Park, {Joo Woong} and Eunji Shin and Choi, {Sung Wook}",

note = "Funding Information: This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning ( NRF-2015R1A4A1042350 and 2017R1A2B4008093 ), a research fund of Biostream Technologies, R&D program of KITECH, the Research Fund, 2018 of The Catholic University of Korea, and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry Health & Welfare, Republic of Korea ( HI17C0886 ). Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

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doi = "10.1016/j.colsurfb.2018.08.003",

language = "English",

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T1 - Fabrication of dihydroxyflavone-conjugated hyaluronic acid nanogels for targeted antitumoral effect

AU - Choi, Yu Ri

AU - Kim, Hyun Jong

AU - Ahn, Guk Young

AU - Lee, Min Jeong

AU - Park, Ju Ri

AU - Jun, Dae Ryong

AU - Ryu, Tae Kyoung

AU - Park, Joo Woong

AU - Shin, Eunji

AU - Choi, Sung Wook

N1 - Funding Information: This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning ( NRF-2015R1A4A1042350 and 2017R1A2B4008093 ), a research fund of Biostream Technologies, R&D program of KITECH, the Research Fund, 2018 of The Catholic University of Korea, and a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry Health & Welfare, Republic of Korea ( HI17C0886 ). Publisher Copyright: © 2018 Elsevier B.V.

PY - 2018/11/1

Y1 - 2018/11/1

N2 - We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels.

AB - We prepared hyaluronic acid (HA)-based nanogels conjugated with dihydroxyflavone (DHF) and evaluated their cellular uptake and antitumoral efficiency. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was used as a conjugation agent for esterification between DHF and HA as well as crosslinking among HA. The conjugations were confirmed by nuclear magnetic resonance spectroscopy, UV/vis spectroscopy, and high-performance liquid chromatography. The size and Zeta-potential of the DHF/HA nanogels were reduced with an increase in the concentration of DMTMM due to the involvement of more HA molecules for the conjugation reactions. The DHF/HA nanogel with a smaller size was greatly taken up by two kinds of tumor cells (HeLa and HepG2), compared to NIH3T3. The cell viabilities were reduced to approximately 60% for HeLa and HepG2 cells after 48 h post treatment with DHF/HA nanogels.

KW - Dihydroxyflavone

KW - Hyaluronic acid

KW - Nanogel

KW - Tumor therapy

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SN - 0927-7765

VL - 171

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Fabrication of dihydroxyflavone-conjugated hyaluronic acid nanogels for targeted antitumoral effect

Abstract

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Keywords

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