Abstract
A polydimethylsiloxane (PDMS) microfluidic device containing a porous membrane as a middle layer was fabricated to continuously produce lipid nanoparticles (LNPs). Ethanol with lipids and water were used as discontinuous and continuous phases. Doxorubicin and lauric acid were added in the ethanol phase for tumor therapy and LNP stability. The smaller LNPs were prepared at the higher flow rate of the continuous phase by using the membrane with smaller pore size. The LNPs with a size of 87.6±3.84 nm were obtained at 0.3 mL/min of the flow rate of the continuous phase by using the membrane with a pore size of 0.02 μm. The addition of lauric acid enhanced the colloidal stability of LNPs. In addition, the LNPs with doxorubicin and lauric acid exhibited lower viability for HeLa cells and enhanced colloidal stability of LNPs. The microfluidic device with a porous membrane can be used as a general platform for the continuous production of LNPs containing mRNA, siRNA, and other therapeutic agents.
| Original language | English |
|---|---|
| Pages (from-to) | 722-727 |
| Number of pages | 6 |
| Journal | Polymer (Korea) |
| Volume | 46 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 2022 |
Bibliographical note
Publisher Copyright:© 2022 The Polymer Society of Korea. All rights reserved.
Keywords
- lipid nanoparticle
- microfluidic
- porous membrane
- tumor therapy