Functional analysis of sigH expression in Corynebacterium glutamicum

Tae Hyun Kim; Hyung Joon Kim; Joon Sung Park; Younhee Kim; Pil Kim; Heung Shick Lee

doi:10.1016/j.bbrc.2005.04.073

Functional analysis of sigH expression in Corynebacterium glutamicum

Tae Hyun Kim
, Hyung Joon Kim
, Joon Sung Park
, Younhee Kim
, Pil Kim
, Heung Shick Lee

Dept. of Biotechnology

Korea University
Semyung University

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

The sigH gene of Corynebacterium glutamicum encodes ECF sigma factor σ^H. The gene apparently plays an important role in other stress responses as well as heat stress response. In this study, we found that deleting the sigH gene made C. glutamicum cells sensitive to the thiol-specific oxidant diamide. In the sigH mutant strain, the activity of thioredoxin reductase markedly decreased, suggesting that the trxB gene encoding thioredoxin reductase is probably under the control of σ^H. The expression of sigH was stimulated in the stationary growth phase and modulated by diamide. In addition, the SigH protein was required for the expression of its own gene. These data indicate that the sigH gene of C. glutamicum stimulates and regulates its own expression in the stationary growth phase in response to environmental stimuli, and participates in the expression of other genes which are important for survival following heat and oxidative stress response.

Original language	English
Pages (from-to)	1542-1547
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	331
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2005.04.073
State	Published - 17 Jun 2005

Bibliographical note

Funding Information:
This work was supported by a grant (R01-2004-000-10093-0 to H.-S. Lee) from the Korean Ministry of Science and Technology (Basic Research Program of the Korea Science and Engineering Foundation) and, in part, by a grant from BASF Korea.

Keywords

Corynebacterium glutamicum
Diamide
Heat stress
Oxidative stress
sigH
trxB

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10.1016/j.bbrc.2005.04.073

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@article{eb3a24bbbd7040459d024ae313355b1d,

title = "Functional analysis of sigH expression in Corynebacterium glutamicum",

abstract = "The sigH gene of Corynebacterium glutamicum encodes ECF sigma factor σH. The gene apparently plays an important role in other stress responses as well as heat stress response. In this study, we found that deleting the sigH gene made C. glutamicum cells sensitive to the thiol-specific oxidant diamide. In the sigH mutant strain, the activity of thioredoxin reductase markedly decreased, suggesting that the trxB gene encoding thioredoxin reductase is probably under the control of σH. The expression of sigH was stimulated in the stationary growth phase and modulated by diamide. In addition, the SigH protein was required for the expression of its own gene. These data indicate that the sigH gene of C. glutamicum stimulates and regulates its own expression in the stationary growth phase in response to environmental stimuli, and participates in the expression of other genes which are important for survival following heat and oxidative stress response.",

keywords = "Corynebacterium glutamicum, Diamide, Heat stress, Oxidative stress, sigH, trxB",

author = "Kim, \{Tae Hyun\} and Kim, \{Hyung Joon\} and Park, \{Joon Sung\} and Younhee Kim and Pil Kim and Lee, \{Heung Shick\}",

note = "Funding Information: This work was supported by a grant (R01-2004-000-10093-0 to H.-S. Lee) from the Korean Ministry of Science and Technology (Basic Research Program of the Korea Science and Engineering Foundation) and, in part, by a grant from BASF Korea.",

year = "2005",

month = jun,

day = "17",

doi = "10.1016/j.bbrc.2005.04.073",

language = "English",

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T1 - Functional analysis of sigH expression in Corynebacterium glutamicum

AU - Kim, Tae Hyun

AU - Kim, Hyung Joon

AU - Park, Joon Sung

AU - Kim, Younhee

AU - Kim, Pil

AU - Lee, Heung Shick

N1 - Funding Information: This work was supported by a grant (R01-2004-000-10093-0 to H.-S. Lee) from the Korean Ministry of Science and Technology (Basic Research Program of the Korea Science and Engineering Foundation) and, in part, by a grant from BASF Korea.

PY - 2005/6/17

Y1 - 2005/6/17

N2 - The sigH gene of Corynebacterium glutamicum encodes ECF sigma factor σH. The gene apparently plays an important role in other stress responses as well as heat stress response. In this study, we found that deleting the sigH gene made C. glutamicum cells sensitive to the thiol-specific oxidant diamide. In the sigH mutant strain, the activity of thioredoxin reductase markedly decreased, suggesting that the trxB gene encoding thioredoxin reductase is probably under the control of σH. The expression of sigH was stimulated in the stationary growth phase and modulated by diamide. In addition, the SigH protein was required for the expression of its own gene. These data indicate that the sigH gene of C. glutamicum stimulates and regulates its own expression in the stationary growth phase in response to environmental stimuli, and participates in the expression of other genes which are important for survival following heat and oxidative stress response.

AB - The sigH gene of Corynebacterium glutamicum encodes ECF sigma factor σH. The gene apparently plays an important role in other stress responses as well as heat stress response. In this study, we found that deleting the sigH gene made C. glutamicum cells sensitive to the thiol-specific oxidant diamide. In the sigH mutant strain, the activity of thioredoxin reductase markedly decreased, suggesting that the trxB gene encoding thioredoxin reductase is probably under the control of σH. The expression of sigH was stimulated in the stationary growth phase and modulated by diamide. In addition, the SigH protein was required for the expression of its own gene. These data indicate that the sigH gene of C. glutamicum stimulates and regulates its own expression in the stationary growth phase in response to environmental stimuli, and participates in the expression of other genes which are important for survival following heat and oxidative stress response.

KW - Corynebacterium glutamicum

KW - Diamide

KW - Heat stress

KW - Oxidative stress

KW - sigH

KW - trxB

UR - https://www.scopus.com/pages/publications/18844432533

U2 - 10.1016/j.bbrc.2005.04.073

DO - 10.1016/j.bbrc.2005.04.073

M3 - Article

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AN - SCOPUS:18844432533

SN - 0006-291X

VL - 331

SP - 1542

EP - 1547

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Functional analysis of sigH expression in Corynebacterium glutamicum

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