Functional identification of the pro-apoptotic effector domain in human Sox4

Eun Hye Hur; Wonhee Hur; Ju Youn Choi; In Kyung Kim; Ho Youn Kim; Seung Kew Yoon; Hyangshuk Rhim

doi:10.1016/j.bbrc.2004.09.215

Functional identification of the pro-apoptotic effector domain in human Sox4

Eun Hye Hur, Wonhee Hur, Ju Youn Choi, In Kyung Kim, Ho Youn Kim, Seung Kew Yoon, Hyangshuk Rhim

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Recent studies provide evidence that Sox4 is involved in regulating apoptosis as well as tumorigenesis of various human cancers; however, its role in the apoptotic machinery is not fully understood. Here we describe that the central domain containing glycine-rich region in Sox4, named CD, is a pivotal pro-apoptotic domain to induce apoptotic cell death. Deletion of the DNA-binding domain or trans-activation domain in Sox4 did not significantly affect pro-apoptotic activity, whereas transient transfection of the high mobility group box or the serine-rich region abrogated the apoptotic activity. Moreover, overexpression of the CD construct (aa 166-342) revealed the apoptotic activity comparable to that of wild-type Sox4, approximately 60% of cell death. Our data suggest that the apoptotic activity of Sox4 can be dissociated from its transcriptional trans-activation and is mediated through its CD.

Original language	English
Pages (from-to)	59-67
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	325
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2004.09.215
State	Published - 3 Dec 2004

Bibliographical note

Funding Information:
We thank H. Clevers for the pCDM7-Sox4 plasmid. This study was supported by the Korean Research Foundation (KRF-99-041-D000344), the 2002 Specialized Research Center grant from the Korean Science and Engineering Foundation to the Rheumatism Research Center at The Catholic University of Korea (R11-2002-098-04003-0), and Korea Institute of Science and Technology Evaluation and Planning and Ministry of Science and Technology, Korean government, through its National Nuclear Technology Program (2004).

Keywords

Apoptosis
DNA-binding domain
Glycine-rich region
High mobility group
Serine-rich region
Sox4
trans-Activation domain

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2004.09.215

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title = "Functional identification of the pro-apoptotic effector domain in human Sox4",

abstract = "Recent studies provide evidence that Sox4 is involved in regulating apoptosis as well as tumorigenesis of various human cancers; however, its role in the apoptotic machinery is not fully understood. Here we describe that the central domain containing glycine-rich region in Sox4, named CD, is a pivotal pro-apoptotic domain to induce apoptotic cell death. Deletion of the DNA-binding domain or trans-activation domain in Sox4 did not significantly affect pro-apoptotic activity, whereas transient transfection of the high mobility group box or the serine-rich region abrogated the apoptotic activity. Moreover, overexpression of the CD construct (aa 166-342) revealed the apoptotic activity comparable to that of wild-type Sox4, approximately 60% of cell death. Our data suggest that the apoptotic activity of Sox4 can be dissociated from its transcriptional trans-activation and is mediated through its CD.",

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note = "Funding Information: We thank H. Clevers for the pCDM7-Sox4 plasmid. This study was supported by the Korean Research Foundation (KRF-99-041-D000344), the 2002 Specialized Research Center grant from the Korean Science and Engineering Foundation to the Rheumatism Research Center at The Catholic University of Korea (R11-2002-098-04003-0), and Korea Institute of Science and Technology Evaluation and Planning and Ministry of Science and Technology, Korean government, through its National Nuclear Technology Program (2004).",

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AU - Hur, Eun Hye

AU - Hur, Wonhee

AU - Choi, Ju Youn

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AU - Kim, Ho Youn

AU - Yoon, Seung Kew

AU - Rhim, Hyangshuk

N1 - Funding Information: We thank H. Clevers for the pCDM7-Sox4 plasmid. This study was supported by the Korean Research Foundation (KRF-99-041-D000344), the 2002 Specialized Research Center grant from the Korean Science and Engineering Foundation to the Rheumatism Research Center at The Catholic University of Korea (R11-2002-098-04003-0), and Korea Institute of Science and Technology Evaluation and Planning and Ministry of Science and Technology, Korean government, through its National Nuclear Technology Program (2004).

PY - 2004/12/3

Y1 - 2004/12/3

N2 - Recent studies provide evidence that Sox4 is involved in regulating apoptosis as well as tumorigenesis of various human cancers; however, its role in the apoptotic machinery is not fully understood. Here we describe that the central domain containing glycine-rich region in Sox4, named CD, is a pivotal pro-apoptotic domain to induce apoptotic cell death. Deletion of the DNA-binding domain or trans-activation domain in Sox4 did not significantly affect pro-apoptotic activity, whereas transient transfection of the high mobility group box or the serine-rich region abrogated the apoptotic activity. Moreover, overexpression of the CD construct (aa 166-342) revealed the apoptotic activity comparable to that of wild-type Sox4, approximately 60% of cell death. Our data suggest that the apoptotic activity of Sox4 can be dissociated from its transcriptional trans-activation and is mediated through its CD.

AB - Recent studies provide evidence that Sox4 is involved in regulating apoptosis as well as tumorigenesis of various human cancers; however, its role in the apoptotic machinery is not fully understood. Here we describe that the central domain containing glycine-rich region in Sox4, named CD, is a pivotal pro-apoptotic domain to induce apoptotic cell death. Deletion of the DNA-binding domain or trans-activation domain in Sox4 did not significantly affect pro-apoptotic activity, whereas transient transfection of the high mobility group box or the serine-rich region abrogated the apoptotic activity. Moreover, overexpression of the CD construct (aa 166-342) revealed the apoptotic activity comparable to that of wild-type Sox4, approximately 60% of cell death. Our data suggest that the apoptotic activity of Sox4 can be dissociated from its transcriptional trans-activation and is mediated through its CD.

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Functional identification of the pro-apoptotic effector domain in human Sox4

Abstract

Bibliographical note

Keywords

UN SDGs

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