Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease

Kee Pyo Kim; Juyong Yoon; Borami Shin; Albrecht Röpke; Dong Wook Han; Hans R. Schöler

doi:10.1016/j.scr.2020.101839

Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease

Kee Pyo Kim
, Juyong Yoon
, Borami Shin
, Albrecht Röpke
, Dong Wook Han
, Hans R. Schöler

Department of Biomedicine & Health Science

Max Planck Institute for Molecular Biomedicine
University of Münster
Wuyi University, Guangdong

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We established a human induced pluripotent stem cells (hiPSC) line (MPIi006-A) from fibroblasts of a 20-year-old male Pelizaeus-Merzbacher disease (PMD) patient with a hemizygous 643C>T mutation in proteolipid protein 1 (PLP1) gene using a retroviral delivery of OCT4, SOX2, KLF4 and c-MYC. The MPIi006-A iPSC line carried the mutation, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited normal karyotype and were capable of differentiating into cells representative of three germ layers.

Original language	English
Article number	101839
Journal	Stem Cell Research
Volume	46
DOIs	https://doi.org/10.1016/j.scr.2020.101839
State	Published - Jul 2020

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© 2020 The Authors

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10.1016/j.scr.2020.101839

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title = "Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease",

abstract = "We established a human induced pluripotent stem cells (hiPSC) line (MPIi006-A) from fibroblasts of a 20-year-old male Pelizaeus-Merzbacher disease (PMD) patient with a hemizygous 643C>T mutation in proteolipid protein 1 (PLP1) gene using a retroviral delivery of OCT4, SOX2, KLF4 and c-MYC. The MPIi006-A iPSC line carried the mutation, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited normal karyotype and were capable of differentiating into cells representative of three germ layers.",

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AU - Kim, Kee Pyo

AU - Yoon, Juyong

AU - Shin, Borami

AU - Röpke, Albrecht

AU - Han, Dong Wook

AU - Schöler, Hans R.

PY - 2020/7

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AB - We established a human induced pluripotent stem cells (hiPSC) line (MPIi006-A) from fibroblasts of a 20-year-old male Pelizaeus-Merzbacher disease (PMD) patient with a hemizygous 643C>T mutation in proteolipid protein 1 (PLP1) gene using a retroviral delivery of OCT4, SOX2, KLF4 and c-MYC. The MPIi006-A iPSC line carried the mutation, displayed typical iPSC morphology, expressed pluripotent stem cell makers, exhibited normal karyotype and were capable of differentiating into cells representative of three germ layers.

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ER -

Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease

Abstract

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