Generation of green fluorescent protein reporter knock-in iPSC line at the 3′UTR region of the KLOTHO locus

Sun Woo Lim; Kang In Lee; Sheng Cui; Xianying Fang; Yoo Jin Shin; Hanbi Lee; Jae Young Lee; Byung Ha Chung; Chul Woo Yang

doi:10.1016/j.scr.2024.103499

Generation of green fluorescent protein reporter knock-in iPSC line at the 3′UTR region of the KLOTHO locus

Sun Woo Lim
, Kang In Lee
, Sheng Cui
, Xianying Fang
, Yoo Jin Shin
, Hanbi Lee
, Jae Young Lee
, Byung Ha Chung
, Chul Woo Yang

The Catholic University of Korea
ToolGen Inc
Catholic Univ. of Korea Coll. Med.

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We generated a human induced pluripotent stem cell (hiPSC) line (CMCi014-A-78) expressing a GFP reporter in the 3′-UTR region of the KLOTHO locus using CRISPR/Cas9-mediated homologous recombination to screen for candidates regulating KLOTHO. The established cell line exhibits a normal karyotype, typical stem cell morphology, expression of pluripotency markers, and the ability to differentiate into the three germ layers. Consequently, this hiPSC line could serve as a valuable resource for screening KLOTHO regulators in hiPSC-derived target cells or organoids.

Original language	English
Article number	103499
Journal	Stem Cell Research
Volume	80
DOIs	https://doi.org/10.1016/j.scr.2024.103499
State	Published - Oct 2024

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© 2024

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title = "Generation of green fluorescent protein reporter knock-in iPSC line at the 3′UTR region of the KLOTHO locus",

abstract = "We generated a human induced pluripotent stem cell (hiPSC) line (CMCi014-A-78) expressing a GFP reporter in the 3′-UTR region of the KLOTHO locus using CRISPR/Cas9-mediated homologous recombination to screen for candidates regulating KLOTHO. The established cell line exhibits a normal karyotype, typical stem cell morphology, expression of pluripotency markers, and the ability to differentiate into the three germ layers. Consequently, this hiPSC line could serve as a valuable resource for screening KLOTHO regulators in hiPSC-derived target cells or organoids.",

author = "Lim, \{Sun Woo\} and Lee, \{Kang In\} and Sheng Cui and Xianying Fang and Shin, \{Yoo Jin\} and Hanbi Lee and Lee, \{Jae Young\} and Chung, \{Byung Ha\} and Yang, \{Chul Woo\}",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2024",

month = oct,

doi = "10.1016/j.scr.2024.103499",

language = "English",

volume = "80",

journal = "Stem Cell Research",

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T1 - Generation of green fluorescent protein reporter knock-in iPSC line at the 3′UTR region of the KLOTHO locus

AU - Lim, Sun Woo

AU - Lee, Kang In

AU - Cui, Sheng

AU - Fang, Xianying

AU - Shin, Yoo Jin

AU - Lee, Hanbi

AU - Lee, Jae Young

AU - Chung, Byung Ha

AU - Yang, Chul Woo

PY - 2024/10

Y1 - 2024/10

N2 - We generated a human induced pluripotent stem cell (hiPSC) line (CMCi014-A-78) expressing a GFP reporter in the 3′-UTR region of the KLOTHO locus using CRISPR/Cas9-mediated homologous recombination to screen for candidates regulating KLOTHO. The established cell line exhibits a normal karyotype, typical stem cell morphology, expression of pluripotency markers, and the ability to differentiate into the three germ layers. Consequently, this hiPSC line could serve as a valuable resource for screening KLOTHO regulators in hiPSC-derived target cells or organoids.

AB - We generated a human induced pluripotent stem cell (hiPSC) line (CMCi014-A-78) expressing a GFP reporter in the 3′-UTR region of the KLOTHO locus using CRISPR/Cas9-mediated homologous recombination to screen for candidates regulating KLOTHO. The established cell line exhibits a normal karyotype, typical stem cell morphology, expression of pluripotency markers, and the ability to differentiate into the three germ layers. Consequently, this hiPSC line could serve as a valuable resource for screening KLOTHO regulators in hiPSC-derived target cells or organoids.

UR - https://www.scopus.com/pages/publications/85200365911

U2 - 10.1016/j.scr.2024.103499

DO - 10.1016/j.scr.2024.103499

M3 - Article

C2 - 39111000

AN - SCOPUS:85200365911

SN - 1873-5061

VL - 80

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 103499

ER -

Generation of green fluorescent protein reporter knock-in iPSC line at the 3′UTR region of the KLOTHO locus

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