High expression of Snail mRNA in blood from hepatocellular carcinoma patients with extra-hepatic metastasis

Ae Lyoung Min; Jong Young Choi; Hyun Young Woo; Jin Dong Kim; Jung Hyun Kwon; Si Hyun Bae; Seung Kew Yoon; Seung Hun Shin; Yeun Jun Chung; Chan Kwon Jung

doi:10.1007/s10585-009-9275-6

High expression of Snail mRNA in blood from hepatocellular carcinoma patients with extra-hepatic metastasis

Ae Lyoung Min
, Jong Young Choi
, Hyun Young Woo
, Jin Dong Kim
, Jung Hyun Kwon
, Si Hyun Bae
, Seung Kew Yoon
, Seung Hun Shin
, Yeun Jun Chung
, Chan Kwon Jung

The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The presence of circulating tumor cells (CTCs) in patients with hepatocellular carcinoma (HCC) suggests metastasis to extra-hepatic organs. Snail is a key regulator of epithelial mesenchymal transition, which is closely associated with tumor metastasis. The aim of this study was to investigate the presence of CTCs and evaluate the significance of Snail mRNA levels in peripheral blood of HCC patients with and without extra-hepatic metastasis. Sixty-six consecutive patients with HCC (30 without metastasis, 36 with metastasis) were prospectively enrolled, as were 30 with liver cirrhosis and 23 healthy subjects. CTCs were isolated by FACS using Ber-EP4 and anti-CD45 antibodies, and CTC identity confirmed by immunofluorescent cytokeratin staining. Snail mRNA levels were measured by quantitative real-time PCR of blood samples. CTCs, positive for pan-cytokeratin and Snail, were isolated from five HCC patients with metastasis. The mean amount of Snail mRNA in HCC with metastasis was 18.8-fold, 26.6-fold greater than HCC without metastasis, liver cirrhosis, respectively. When compared with healthy controls, the mean level of Snail mRNA in HCC without metastasis was 10.1-fold greater (P < 0.001). In six patients showing complete remission of HCC, Snail mRNA decreased to levels similar to those of healthy controls. This study suggests the possibility that circulating Snail mRNA levels may have been associated with extra-hepatic metastasis in HCC patients.

Original language	English
Pages (from-to)	759-767
Number of pages	9
Journal	Clinical and Experimental Metastasis
Volume	26
Issue number	7
DOIs	https://doi.org/10.1007/s10585-009-9275-6
State	Published - Oct 2009

Bibliographical note

Funding Information:
Acknowledgments This study was supported in part by grant of the Leading Foreign Research Institute Recruitment Program and from FG06-12-01 of the 21C Frontier Functional Human Genome Project from the Ministry of Science & Technology in Korea.

Keywords

Circulating tumor cell
Hepatocellular Carcinoma
Metastasis
Snail

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10585-009-9275-6

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title = "High expression of Snail mRNA in blood from hepatocellular carcinoma patients with extra-hepatic metastasis",

abstract = "The presence of circulating tumor cells (CTCs) in patients with hepatocellular carcinoma (HCC) suggests metastasis to extra-hepatic organs. Snail is a key regulator of epithelial mesenchymal transition, which is closely associated with tumor metastasis. The aim of this study was to investigate the presence of CTCs and evaluate the significance of Snail mRNA levels in peripheral blood of HCC patients with and without extra-hepatic metastasis. Sixty-six consecutive patients with HCC (30 without metastasis, 36 with metastasis) were prospectively enrolled, as were 30 with liver cirrhosis and 23 healthy subjects. CTCs were isolated by FACS using Ber-EP4 and anti-CD45 antibodies, and CTC identity confirmed by immunofluorescent cytokeratin staining. Snail mRNA levels were measured by quantitative real-time PCR of blood samples. CTCs, positive for pan-cytokeratin and Snail, were isolated from five HCC patients with metastasis. The mean amount of Snail mRNA in HCC with metastasis was 18.8-fold, 26.6-fold greater than HCC without metastasis, liver cirrhosis, respectively. When compared with healthy controls, the mean level of Snail mRNA in HCC without metastasis was 10.1-fold greater (P < 0.001). In six patients showing complete remission of HCC, Snail mRNA decreased to levels similar to those of healthy controls. This study suggests the possibility that circulating Snail mRNA levels may have been associated with extra-hepatic metastasis in HCC patients.",

keywords = "Circulating tumor cell, Hepatocellular Carcinoma, Metastasis, Snail",

author = "Min, \{Ae Lyoung\} and Choi, \{Jong Young\} and Woo, \{Hyun Young\} and Kim, \{Jin Dong\} and Kwon, \{Jung Hyun\} and Bae, \{Si Hyun\} and Yoon, \{Seung Kew\} and Shin, \{Seung Hun\} and Chung, \{Yeun Jun\} and Jung, \{Chan Kwon\}",

note = "Funding Information: Acknowledgments This study was supported in part by grant of the Leading Foreign Research Institute Recruitment Program and from FG06-12-01 of the 21C Frontier Functional Human Genome Project from the Ministry of Science \& Technology in Korea.",

year = "2009",

month = oct,

doi = "10.1007/s10585-009-9275-6",

language = "English",

volume = "26",

pages = "759--767",

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T1 - High expression of Snail mRNA in blood from hepatocellular carcinoma patients with extra-hepatic metastasis

AU - Min, Ae Lyoung

AU - Choi, Jong Young

AU - Woo, Hyun Young

AU - Kim, Jin Dong

AU - Kwon, Jung Hyun

AU - Bae, Si Hyun

AU - Yoon, Seung Kew

AU - Shin, Seung Hun

AU - Chung, Yeun Jun

AU - Jung, Chan Kwon

N1 - Funding Information: Acknowledgments This study was supported in part by grant of the Leading Foreign Research Institute Recruitment Program and from FG06-12-01 of the 21C Frontier Functional Human Genome Project from the Ministry of Science & Technology in Korea.

PY - 2009/10

Y1 - 2009/10

N2 - The presence of circulating tumor cells (CTCs) in patients with hepatocellular carcinoma (HCC) suggests metastasis to extra-hepatic organs. Snail is a key regulator of epithelial mesenchymal transition, which is closely associated with tumor metastasis. The aim of this study was to investigate the presence of CTCs and evaluate the significance of Snail mRNA levels in peripheral blood of HCC patients with and without extra-hepatic metastasis. Sixty-six consecutive patients with HCC (30 without metastasis, 36 with metastasis) were prospectively enrolled, as were 30 with liver cirrhosis and 23 healthy subjects. CTCs were isolated by FACS using Ber-EP4 and anti-CD45 antibodies, and CTC identity confirmed by immunofluorescent cytokeratin staining. Snail mRNA levels were measured by quantitative real-time PCR of blood samples. CTCs, positive for pan-cytokeratin and Snail, were isolated from five HCC patients with metastasis. The mean amount of Snail mRNA in HCC with metastasis was 18.8-fold, 26.6-fold greater than HCC without metastasis, liver cirrhosis, respectively. When compared with healthy controls, the mean level of Snail mRNA in HCC without metastasis was 10.1-fold greater (P < 0.001). In six patients showing complete remission of HCC, Snail mRNA decreased to levels similar to those of healthy controls. This study suggests the possibility that circulating Snail mRNA levels may have been associated with extra-hepatic metastasis in HCC patients.

AB - The presence of circulating tumor cells (CTCs) in patients with hepatocellular carcinoma (HCC) suggests metastasis to extra-hepatic organs. Snail is a key regulator of epithelial mesenchymal transition, which is closely associated with tumor metastasis. The aim of this study was to investigate the presence of CTCs and evaluate the significance of Snail mRNA levels in peripheral blood of HCC patients with and without extra-hepatic metastasis. Sixty-six consecutive patients with HCC (30 without metastasis, 36 with metastasis) were prospectively enrolled, as were 30 with liver cirrhosis and 23 healthy subjects. CTCs were isolated by FACS using Ber-EP4 and anti-CD45 antibodies, and CTC identity confirmed by immunofluorescent cytokeratin staining. Snail mRNA levels were measured by quantitative real-time PCR of blood samples. CTCs, positive for pan-cytokeratin and Snail, were isolated from five HCC patients with metastasis. The mean amount of Snail mRNA in HCC with metastasis was 18.8-fold, 26.6-fold greater than HCC without metastasis, liver cirrhosis, respectively. When compared with healthy controls, the mean level of Snail mRNA in HCC without metastasis was 10.1-fold greater (P < 0.001). In six patients showing complete remission of HCC, Snail mRNA decreased to levels similar to those of healthy controls. This study suggests the possibility that circulating Snail mRNA levels may have been associated with extra-hepatic metastasis in HCC patients.

KW - Circulating tumor cell

KW - Hepatocellular Carcinoma

KW - Metastasis

KW - Snail

UR - https://www.scopus.com/pages/publications/71849093192

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DO - 10.1007/s10585-009-9275-6

M3 - Article

C2 - 19533388

AN - SCOPUS:71849093192

SN - 0262-0898

VL - 26

SP - 759

EP - 767

JO - Clinical and Experimental Metastasis

JF - Clinical and Experimental Metastasis

IS - 7

ER -

High expression of Snail mRNA in blood from hepatocellular carcinoma patients with extra-hepatic metastasis

Abstract

Bibliographical note

Keywords

UN SDGs

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