Human melanoma cells adhesion on the surface of polymeric chemoattractants

Two dimensional(2-D) polymeric matrices with free radical generating quinoid compounds, hydro-quinone-grafted-polypropylene(HQ-g-PP) and vitamin-K₁-grafted-polypropylene(VK₁-g-PP), were prepared by a liquid phase photo-grafting technique, and the adhesion of primary(WM 793) and metastatic(WM 1205) human melanoma cells to prepared surfaces was investigated. Grafting of polar groups in VK₁and HQ increased surface tension of PP matrices from 27 dyne/cm to 39 dyne/cm, resulting in decreased protein adsorption. In addition, electron paramagnetic resonance(EPR) spectra showed that the matrices generated free radicals at the interface when in contact with serum. Both WM 793 and WM 1205 cells showed an enhanced adhesion to the prepared samples compared to the control PP. The adhesion of metastatic melanoma cells onto the polymeric matrices was more pronounced than that of primary melanoma cells. VK₁-g-PP with twice as many free radical generating groups in a unit graft yield as HQ-g-PP had a greater cell adhesion. The number of cells adhered to the surface of the polymeric chemoattractants increases with increase in the content of free radicals at the interface.