Identification and characterization of SMI32-immunoreactive amacrine cells in the mouse retina

Eun Jin Lim; In Beom Kim; Su Ja Oh; Myung Hoon Chun

doi:10.1016/j.neulet.2007.07.046

Identification and characterization of SMI32-immunoreactive amacrine cells in the mouse retina

Eun Jin Lim, In Beom Kim, Su Ja Oh, Myung Hoon Chun

Department of Anatomy

The Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Mammalian neurons express the neural intermediate filament protein neurofilament (NF). In the retina, NFs have been detected primarily in the axons and processes of retinal ganglion and horizontal cells. We found an amacrine cell type that was immunolabeled with an antibody against SMI32, a non-phosphorylated epitope on neurofilament proteins of high molecular weight, in the mouse retina. This type of amacrine cell was non-randomly distributed, and these cells exhibited a central-peripheral density gradient. Most of these cells co-expressed GABA and ChAT, but not glycine or any other amacrine cell marker. These results suggest that some SMI32-immunoreactive amacrine cells belong to a GABAergic population, and that SMI32 can therefore be used as a marker for a subset of amacrine cells in addition to ganglion cells and horizontal cells in the mouse retina.

Original language	English
Pages (from-to)	199-202
Number of pages	4
Journal	Neuroscience Letters
Volume	424
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2007.07.046
State	Published - 13 Sep 2007

Bibliographical note

Funding Information:
Supported by Neurobiology Support Grant (M1-0108-00-0059) of the Ministry of Science and Technology, Korea.

Keywords

Amacrine cell
Mouse
Neurofilaments
Retina
SMI32

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10.1016/j.neulet.2007.07.046

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title = "Identification and characterization of SMI32-immunoreactive amacrine cells in the mouse retina",

abstract = "Mammalian neurons express the neural intermediate filament protein neurofilament (NF). In the retina, NFs have been detected primarily in the axons and processes of retinal ganglion and horizontal cells. We found an amacrine cell type that was immunolabeled with an antibody against SMI32, a non-phosphorylated epitope on neurofilament proteins of high molecular weight, in the mouse retina. This type of amacrine cell was non-randomly distributed, and these cells exhibited a central-peripheral density gradient. Most of these cells co-expressed GABA and ChAT, but not glycine or any other amacrine cell marker. These results suggest that some SMI32-immunoreactive amacrine cells belong to a GABAergic population, and that SMI32 can therefore be used as a marker for a subset of amacrine cells in addition to ganglion cells and horizontal cells in the mouse retina.",

keywords = "Amacrine cell, Mouse, Neurofilaments, Retina, SMI32",

author = "Lim, {Eun Jin} and Kim, {In Beom} and Oh, {Su Ja} and Chun, {Myung Hoon}",

note = "Funding Information: Supported by Neurobiology Support Grant (M1-0108-00-0059) of the Ministry of Science and Technology, Korea.",

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AU - Lim, Eun Jin

AU - Kim, In Beom

AU - Oh, Su Ja

AU - Chun, Myung Hoon

N1 - Funding Information: Supported by Neurobiology Support Grant (M1-0108-00-0059) of the Ministry of Science and Technology, Korea.

PY - 2007/9/13

Y1 - 2007/9/13

N2 - Mammalian neurons express the neural intermediate filament protein neurofilament (NF). In the retina, NFs have been detected primarily in the axons and processes of retinal ganglion and horizontal cells. We found an amacrine cell type that was immunolabeled with an antibody against SMI32, a non-phosphorylated epitope on neurofilament proteins of high molecular weight, in the mouse retina. This type of amacrine cell was non-randomly distributed, and these cells exhibited a central-peripheral density gradient. Most of these cells co-expressed GABA and ChAT, but not glycine or any other amacrine cell marker. These results suggest that some SMI32-immunoreactive amacrine cells belong to a GABAergic population, and that SMI32 can therefore be used as a marker for a subset of amacrine cells in addition to ganglion cells and horizontal cells in the mouse retina.

AB - Mammalian neurons express the neural intermediate filament protein neurofilament (NF). In the retina, NFs have been detected primarily in the axons and processes of retinal ganglion and horizontal cells. We found an amacrine cell type that was immunolabeled with an antibody against SMI32, a non-phosphorylated epitope on neurofilament proteins of high molecular weight, in the mouse retina. This type of amacrine cell was non-randomly distributed, and these cells exhibited a central-peripheral density gradient. Most of these cells co-expressed GABA and ChAT, but not glycine or any other amacrine cell marker. These results suggest that some SMI32-immunoreactive amacrine cells belong to a GABAergic population, and that SMI32 can therefore be used as a marker for a subset of amacrine cells in addition to ganglion cells and horizontal cells in the mouse retina.

KW - Amacrine cell

KW - Mouse

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Identification and characterization of SMI32-immunoreactive amacrine cells in the mouse retina

Abstract

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Keywords

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