Improvement of tagatose conversion rate by genetic evolution of thermostable galactose isomerase

P. Kim; Hyun Yoon Sang Hyun Yoon; Ji Seo Myung Ji Seo; Kun Oh Deok Kun Oh; Hwan Choi Jin Hwan Choi

doi:10.1042/BA20010025

Improvement of tagatose conversion rate by genetic evolution of thermostable galactose isomerase

P. Kim
, Hyun Yoon Sang Hyun Yoon
, Ji Seo Myung Ji Seo
, Kun Oh Deok Kun Oh
, Hwan Choi Jin Hwan Choi

Dept. of Biotechnology

Sejong University

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

To enhance the isomerization rate of galactose into tagatose, a thermostable galactose isomerase, which was isolated from bacteria growing in a hot spring, was genetically improved using the error-prone PCR method. From 500 mutant clones, a clone showing improved conversion activity was selected. The sequence of the selected clone had five amino acid changes: His²²⁸ → Asp, Gly³⁸⁴ → Asp, Ser³⁹³ → Thr, Lys⁴²⁸ → Asn and Asp⁴⁷⁵ → Lys. The improved galactose isomerase had an II-fold higher reaction rate than the original.

Original language	English
Pages (from-to)	99-102
Number of pages	4
Journal	Biotechnology and Applied Biochemistry
Volume	34
Issue number	2
DOIs	https://doi.org/10.1042/BA20010025
State	Published - 2001

Keywords

Activity enhancement
Error-prone PCR
Isomerization

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10.1042/BA20010025

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title = "Improvement of tagatose conversion rate by genetic evolution of thermostable galactose isomerase",

abstract = "To enhance the isomerization rate of galactose into tagatose, a thermostable galactose isomerase, which was isolated from bacteria growing in a hot spring, was genetically improved using the error-prone PCR method. From 500 mutant clones, a clone showing improved conversion activity was selected. The sequence of the selected clone had five amino acid changes: His228 → Asp, Gly384 → Asp, Ser393 → Thr, Lys428 → Asn and Asp475 → Lys. The improved galactose isomerase had an II-fold higher reaction rate than the original.",

keywords = "Activity enhancement, Error-prone PCR, Isomerization",

author = "P. Kim and \{Sang Hyun Yoon\}, \{Hyun Yoon\} and \{Myung Ji Seo\}, \{Ji Seo\} and \{Deok Kun Oh\}, \{Kun Oh\} and \{Jin Hwan Choi\}, \{Hwan Choi\}",

year = "2001",

doi = "10.1042/BA20010025",

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T1 - Improvement of tagatose conversion rate by genetic evolution of thermostable galactose isomerase

AU - Kim, P.

AU - Sang Hyun Yoon, Hyun Yoon

AU - Myung Ji Seo, Ji Seo

AU - Deok Kun Oh, Kun Oh

AU - Jin Hwan Choi, Hwan Choi

PY - 2001

Y1 - 2001

N2 - To enhance the isomerization rate of galactose into tagatose, a thermostable galactose isomerase, which was isolated from bacteria growing in a hot spring, was genetically improved using the error-prone PCR method. From 500 mutant clones, a clone showing improved conversion activity was selected. The sequence of the selected clone had five amino acid changes: His228 → Asp, Gly384 → Asp, Ser393 → Thr, Lys428 → Asn and Asp475 → Lys. The improved galactose isomerase had an II-fold higher reaction rate than the original.

AB - To enhance the isomerization rate of galactose into tagatose, a thermostable galactose isomerase, which was isolated from bacteria growing in a hot spring, was genetically improved using the error-prone PCR method. From 500 mutant clones, a clone showing improved conversion activity was selected. The sequence of the selected clone had five amino acid changes: His228 → Asp, Gly384 → Asp, Ser393 → Thr, Lys428 → Asn and Asp475 → Lys. The improved galactose isomerase had an II-fold higher reaction rate than the original.

KW - Activity enhancement

KW - Error-prone PCR

KW - Isomerization

UR - https://www.scopus.com/pages/publications/0034775246

U2 - 10.1042/BA20010025

DO - 10.1042/BA20010025

M3 - Article

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AN - SCOPUS:0034775246

SN - 0885-4513

VL - 34

SP - 99

EP - 102

JO - Biotechnology and Applied Biochemistry

JF - Biotechnology and Applied Biochemistry

IS - 2

ER -

Improvement of tagatose conversion rate by genetic evolution of thermostable galactose isomerase

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Keywords

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