Increased ciliary neurotrophic factor expression in reactive astrocytes following spinal cord injury in the rat

Mun Yong Lee; Chang Jae Kim; Soon Lim Shin; Se Ho Moon; Myung Hoon Chun

doi:10.1016/S0304-3940(98)00710-1

Increased ciliary neurotrophic factor expression in reactive astrocytes following spinal cord injury in the rat

Mun Yong Lee, Chang Jae Kim, Soon Lim Shin, Se Ho Moon, Myung Hoon Chun

Department of Anatomy

Daegu Catholic University School of Medicine

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Expression of ciliary neurotrophic factor (CNTF) was observed in reactive astrocytes in injured spinal cord of the adult rat. After unilateral incision of the dorsal funiculus at a midthoracic level, the rats were sacrificed on the day of postoperation (DPO) 3, 7, 14 or 28. Western blot analysis of spinal cord extracts showed that the intensity of the CNTF- immunoreactive band of 24 kDa was increased on DPO 3 and 7 and decreased on DPO 14. CNTF- or glial fibrillary acidic protein (GFAP)-like immunoreactivity was also increased progressively in the injured dorsal funiculus and adjacent gray matter. Double immunofluorescence histochemistry revealed that all CNTF- like immunoreactive cells showed GFAP-like immunoreactivity. The CNTF upregulation in the reactive astrocytes may play important roles in repair process after spinal cord injury.

Original language	English
Pages (from-to)	79-82
Number of pages	4
Journal	Neuroscience Letters
Volume	255
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(98)00710-1
State	Published - 2 Sep 1998

Keywords

Astrocytes
Ciliary neurotrophic factor
Immunocytochemistry
Rat
Spinal cord
Western blotting

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10.1016/S0304-3940(98)00710-1

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title = "Increased ciliary neurotrophic factor expression in reactive astrocytes following spinal cord injury in the rat",

abstract = "Expression of ciliary neurotrophic factor (CNTF) was observed in reactive astrocytes in injured spinal cord of the adult rat. After unilateral incision of the dorsal funiculus at a midthoracic level, the rats were sacrificed on the day of postoperation (DPO) 3, 7, 14 or 28. Western blot analysis of spinal cord extracts showed that the intensity of the CNTF- immunoreactive band of 24 kDa was increased on DPO 3 and 7 and decreased on DPO 14. CNTF- or glial fibrillary acidic protein (GFAP)-like immunoreactivity was also increased progressively in the injured dorsal funiculus and adjacent gray matter. Double immunofluorescence histochemistry revealed that all CNTF- like immunoreactive cells showed GFAP-like immunoreactivity. The CNTF upregulation in the reactive astrocytes may play important roles in repair process after spinal cord injury.",

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AU - Kim, Chang Jae

AU - Shin, Soon Lim

AU - Moon, Se Ho

AU - Chun, Myung Hoon

PY - 1998/9/2

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N2 - Expression of ciliary neurotrophic factor (CNTF) was observed in reactive astrocytes in injured spinal cord of the adult rat. After unilateral incision of the dorsal funiculus at a midthoracic level, the rats were sacrificed on the day of postoperation (DPO) 3, 7, 14 or 28. Western blot analysis of spinal cord extracts showed that the intensity of the CNTF- immunoreactive band of 24 kDa was increased on DPO 3 and 7 and decreased on DPO 14. CNTF- or glial fibrillary acidic protein (GFAP)-like immunoreactivity was also increased progressively in the injured dorsal funiculus and adjacent gray matter. Double immunofluorescence histochemistry revealed that all CNTF- like immunoreactive cells showed GFAP-like immunoreactivity. The CNTF upregulation in the reactive astrocytes may play important roles in repair process after spinal cord injury.

AB - Expression of ciliary neurotrophic factor (CNTF) was observed in reactive astrocytes in injured spinal cord of the adult rat. After unilateral incision of the dorsal funiculus at a midthoracic level, the rats were sacrificed on the day of postoperation (DPO) 3, 7, 14 or 28. Western blot analysis of spinal cord extracts showed that the intensity of the CNTF- immunoreactive band of 24 kDa was increased on DPO 3 and 7 and decreased on DPO 14. CNTF- or glial fibrillary acidic protein (GFAP)-like immunoreactivity was also increased progressively in the injured dorsal funiculus and adjacent gray matter. Double immunofluorescence histochemistry revealed that all CNTF- like immunoreactive cells showed GFAP-like immunoreactivity. The CNTF upregulation in the reactive astrocytes may play important roles in repair process after spinal cord injury.

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KW - Ciliary neurotrophic factor

KW - Immunocytochemistry

KW - Rat

KW - Spinal cord

KW - Western blotting

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Increased ciliary neurotrophic factor expression in reactive astrocytes following spinal cord injury in the rat

Abstract

Keywords

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