Increased expression of ciliary neurotrophic factor receptor α mRNA in the ischemic rat retina

Won Kyu Ju; Mun Yong Lee; Hans Dieter Hofmann; Matthias Kirsch; Su Ja Oh; Jin Woong Chung; Myung Hoon Chun

doi:10.1016/S0304-3940(00)00931-9

Increased expression of ciliary neurotrophic factor receptor α mRNA in the ischemic rat retina

Won Kyu Ju, Mun Yong Lee, Hans Dieter Hofmann, Matthias Kirsch, Su Ja Oh, Jin Woong Chung, Myung Hoon Chun

Department of Anatomy

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Using in situ hybridization, we investigated the expression of ciliary neurotrophic factor receptor ((CNTFRα) mRNA in the rat retina rendered ischemic by elevation of the intraocular pressure (IOP). The IOP was increased to 120 mmHg and maintained for 60 min. The rats were sacrificed on the day of reperfusion (DRP) 1, 3, 7, 14, and 28. In the normal retina, the signal for CNTFRα mRNA was present in retinal cells in the inner nuclear layer (INL) and in the ganglion cell layer (GCL). On DRP 1, numerous cells in the INL and GCL showed a CNTFRα mRNA signal. From DRP 3 onwards, CNTFRα mRNA appeared in photoreceptor cells located in the outer part of the outer nuclear layer. The signal in these cells increased up to DRP 14 and then decreased at DRP 28. Our findings suggest that cells expressing CNTFRα mRNA may resist the degenerative processes induced by ischemic insult in the rat retina. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	133-136
Number of pages	4
Journal	Neuroscience Letters
Volume	283
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(00)00931-9
State	Published - 7 Apr 2000

Bibliographical note

Funding Information:
This work was supported by a grant from the Korean Ministry of Health and Welfare (HMP-96-M-2-1042) and a Roche grant. We thank Hee-Duck Rho and Byoung-Ouk Hong for their excellent technical assistance.

Keywords

Ciliary neurotrophic factor receptor α mRNA
Elevated intraocular pressure
In situ hybridization
Rat
Retina

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10.1016/S0304-3940(00)00931-9

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title = "Increased expression of ciliary neurotrophic factor receptor α mRNA in the ischemic rat retina",

abstract = "Using in situ hybridization, we investigated the expression of ciliary neurotrophic factor receptor ((CNTFRα) mRNA in the rat retina rendered ischemic by elevation of the intraocular pressure (IOP). The IOP was increased to 120 mmHg and maintained for 60 min. The rats were sacrificed on the day of reperfusion (DRP) 1, 3, 7, 14, and 28. In the normal retina, the signal for CNTFRα mRNA was present in retinal cells in the inner nuclear layer (INL) and in the ganglion cell layer (GCL). On DRP 1, numerous cells in the INL and GCL showed a CNTFRα mRNA signal. From DRP 3 onwards, CNTFRα mRNA appeared in photoreceptor cells located in the outer part of the outer nuclear layer. The signal in these cells increased up to DRP 14 and then decreased at DRP 28. Our findings suggest that cells expressing CNTFRα mRNA may resist the degenerative processes induced by ischemic insult in the rat retina. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Ciliary neurotrophic factor receptor α mRNA, Elevated intraocular pressure, In situ hybridization, Rat, Retina",

author = "Ju, {Won Kyu} and Lee, {Mun Yong} and Hofmann, {Hans Dieter} and Matthias Kirsch and Oh, {Su Ja} and Chung, {Jin Woong} and Chun, {Myung Hoon}",

note = "Funding Information: This work was supported by a grant from the Korean Ministry of Health and Welfare (HMP-96-M-2-1042) and a Roche grant. We thank Hee-Duck Rho and Byoung-Ouk Hong for their excellent technical assistance.",

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AU - Lee, Mun Yong

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AU - Kirsch, Matthias

AU - Oh, Su Ja

AU - Chung, Jin Woong

AU - Chun, Myung Hoon

N1 - Funding Information: This work was supported by a grant from the Korean Ministry of Health and Welfare (HMP-96-M-2-1042) and a Roche grant. We thank Hee-Duck Rho and Byoung-Ouk Hong for their excellent technical assistance.

PY - 2000/4/7

Y1 - 2000/4/7

N2 - Using in situ hybridization, we investigated the expression of ciliary neurotrophic factor receptor ((CNTFRα) mRNA in the rat retina rendered ischemic by elevation of the intraocular pressure (IOP). The IOP was increased to 120 mmHg and maintained for 60 min. The rats were sacrificed on the day of reperfusion (DRP) 1, 3, 7, 14, and 28. In the normal retina, the signal for CNTFRα mRNA was present in retinal cells in the inner nuclear layer (INL) and in the ganglion cell layer (GCL). On DRP 1, numerous cells in the INL and GCL showed a CNTFRα mRNA signal. From DRP 3 onwards, CNTFRα mRNA appeared in photoreceptor cells located in the outer part of the outer nuclear layer. The signal in these cells increased up to DRP 14 and then decreased at DRP 28. Our findings suggest that cells expressing CNTFRα mRNA may resist the degenerative processes induced by ischemic insult in the rat retina. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - Using in situ hybridization, we investigated the expression of ciliary neurotrophic factor receptor ((CNTFRα) mRNA in the rat retina rendered ischemic by elevation of the intraocular pressure (IOP). The IOP was increased to 120 mmHg and maintained for 60 min. The rats were sacrificed on the day of reperfusion (DRP) 1, 3, 7, 14, and 28. In the normal retina, the signal for CNTFRα mRNA was present in retinal cells in the inner nuclear layer (INL) and in the ganglion cell layer (GCL). On DRP 1, numerous cells in the INL and GCL showed a CNTFRα mRNA signal. From DRP 3 onwards, CNTFRα mRNA appeared in photoreceptor cells located in the outer part of the outer nuclear layer. The signal in these cells increased up to DRP 14 and then decreased at DRP 28. Our findings suggest that cells expressing CNTFRα mRNA may resist the degenerative processes induced by ischemic insult in the rat retina. Copyright (C) 2000 Elsevier Science Ireland Ltd.

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Increased expression of ciliary neurotrophic factor receptor α mRNA in the ischemic rat retina

Abstract

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Keywords

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