Inhibition of herpesvirus-6B RNA replication by short interference RNAs

Jong Sub Yoon; Sun Hwa Kim; Min Chul Shin; Dong Gun Lee; Seong Karp Hong; Yong Tae Jung; In Gu Khang; Wan Shik Shin; Chun Choo Kim; Soon Young Paik

doi:10.5483/bmbrep.2004.37.3.383

Inhibition of herpesvirus-6B RNA replication by short interference RNAs

Jong Sub Yoon
, Sun Hwa Kim
, Min Chul Shin
, Dong Gun Lee
, Seong Karp Hong
, Yong Tae Jung
, In Gu Khang
, Wan Shik Shin
, Chun Choo Kim
, Soon Young Paik

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

RNA interference (RNAi) is a process of sequence-specific gene silencing, which is initiated by double-stranded RNA (dsRNA). RNAi may also serve as an antiviral system in vertebrates. This study describes the inhibition of herpesvirus-6B (HHV-6B) replication by short interference RNAs (siRNAs) that are targeted to the U38 sequence that encodes DNA polymerase. When virus-infected SupT1 cells were treated by siRNA, these cells blocked the cytopathic effect (CPE) and detected the HHV-6B antibody-negative in indirect immunofluorescence assays (IFA). Our result suggests that RNAi can efficiently block Herpesvirus-6B replication.

Original language	English
Pages (from-to)	383-385
Number of pages	3
Journal	Journal of Biochemistry and Molecular Biology
Volume	37
Issue number	3
DOIs	https://doi.org/10.5483/bmbrep.2004.37.3.383
State	Published - 31 May 2004

Keywords

Herpes virus 6B
RNA interference
SupT1 cells

Access to Document

10.5483/bmbrep.2004.37.3.383

Cite this

@article{b9bc124c54974193b5645ddedab6330c,

title = "Inhibition of herpesvirus-6B RNA replication by short interference RNAs",

abstract = "RNA interference (RNAi) is a process of sequence-specific gene silencing, which is initiated by double-stranded RNA (dsRNA). RNAi may also serve as an antiviral system in vertebrates. This study describes the inhibition of herpesvirus-6B (HHV-6B) replication by short interference RNAs (siRNAs) that are targeted to the U38 sequence that encodes DNA polymerase. When virus-infected SupT1 cells were treated by siRNA, these cells blocked the cytopathic effect (CPE) and detected the HHV-6B antibody-negative in indirect immunofluorescence assays (IFA). Our result suggests that RNAi can efficiently block Herpesvirus-6B replication.",

keywords = "Herpes virus 6B, RNA interference, SupT1 cells",

author = "Yoon, \{Jong Sub\} and Kim, \{Sun Hwa\} and Shin, \{Min Chul\} and Lee, \{Dong Gun\} and Hong, \{Seong Karp\} and Jung, \{Yong Tae\} and Khang, \{In Gu\} and Shin, \{Wan Shik\} and Kim, \{Chun Choo\} and Paik, \{Soon Young\}",

year = "2004",

month = may,

day = "31",

doi = "10.5483/bmbrep.2004.37.3.383",

language = "English",

volume = "37",

pages = "383--385",

journal = "Journal of Biochemistry and Molecular Biology",

issn = "1225-8687",

publisher = "The Biochemical Society of the Republic of Korea",

number = "3",

}

TY - JOUR

T1 - Inhibition of herpesvirus-6B RNA replication by short interference RNAs

AU - Yoon, Jong Sub

AU - Kim, Sun Hwa

AU - Shin, Min Chul

AU - Lee, Dong Gun

AU - Hong, Seong Karp

AU - Jung, Yong Tae

AU - Khang, In Gu

AU - Shin, Wan Shik

AU - Kim, Chun Choo

AU - Paik, Soon Young

PY - 2004/5/31

Y1 - 2004/5/31

N2 - RNA interference (RNAi) is a process of sequence-specific gene silencing, which is initiated by double-stranded RNA (dsRNA). RNAi may also serve as an antiviral system in vertebrates. This study describes the inhibition of herpesvirus-6B (HHV-6B) replication by short interference RNAs (siRNAs) that are targeted to the U38 sequence that encodes DNA polymerase. When virus-infected SupT1 cells were treated by siRNA, these cells blocked the cytopathic effect (CPE) and detected the HHV-6B antibody-negative in indirect immunofluorescence assays (IFA). Our result suggests that RNAi can efficiently block Herpesvirus-6B replication.

AB - RNA interference (RNAi) is a process of sequence-specific gene silencing, which is initiated by double-stranded RNA (dsRNA). RNAi may also serve as an antiviral system in vertebrates. This study describes the inhibition of herpesvirus-6B (HHV-6B) replication by short interference RNAs (siRNAs) that are targeted to the U38 sequence that encodes DNA polymerase. When virus-infected SupT1 cells were treated by siRNA, these cells blocked the cytopathic effect (CPE) and detected the HHV-6B antibody-negative in indirect immunofluorescence assays (IFA). Our result suggests that RNAi can efficiently block Herpesvirus-6B replication.

KW - Herpes virus 6B

KW - RNA interference

KW - SupT1 cells

UR - https://www.scopus.com/pages/publications/3142758485

U2 - 10.5483/bmbrep.2004.37.3.383

DO - 10.5483/bmbrep.2004.37.3.383

M3 - Article

C2 - 15469723

AN - SCOPUS:3142758485

SN - 1225-8687

VL - 37

SP - 383

EP - 385

JO - Journal of Biochemistry and Molecular Biology

JF - Journal of Biochemistry and Molecular Biology

IS - 3

ER -

Inhibition of herpesvirus-6B RNA replication by short interference RNAs

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this