Integrated biofabrication of artificial esophageal scaffolds using electrospinning, embedded DLP, and extrusion techniques

Esophageal reconstruction faces critical challenges due to limitations in current techniques, including inadequate mechanical properties, poor tissue integration, and insufficient functional regeneration. This study presents a novel biofabrication strategy for developing artificial esophageal scaffolds by integrating electrospinning, embedded digital light processing (DLP), and extrusion-based bioprinting techniques. These scaffolds are composed of flexible electrospun polyurethane (PU) nanofibers wherein silk fibroin methacryloyl (Sil-MA) is embedded within the PU layer to enhance mechanical strength and hydrophilicity. Decellularized esophageal extracellular matrix (EdECM) is deposited onto the scaffolds to promote tissue regeneration. Comprehensive in vitro and in vivo evaluations reveal that the PU/Sil-MA/EdECM scaffolds exhibit superior mechanical properties, enhanced cell adhesion, and significant improvements in smooth muscle and epithelial tissue regeneration. Moreover, in a rat model with partial esophageal defects, the scaffolds demonstrate successful tissue integration, reduced postoperative complications, and restoration of esophageal function, including peristalsis and nerve regeneration. Altogether, this integrated biofabrication approach offers a promising solution for esophageal reconstruction by effectively addressing the current challenges and paving the way for future clinical applications in regenerative medicine.