Abstract
Y-box binding protein 1 (YB-1) is a member of the cold-shock domain (CSD) protein superfamily. It participates in a wide variety of cellular events, including transcription, RNA splicing, translation, DNA repair, drug resistance, and stress responses. We investigated putative functions of YB-1 in HIV-1 replication. Functional studies using overexpression or knockdown of YB-1 in conjunction with transfection of proviral DNA showed that YB-1 enhances virus production. We found YB-1 regulates HIV-1 production by stimulating viral transcription using HIV-1 LTR sequence U3RU5 with Luciferase assay. We also identified a specific region from amino acids 1 to 324 of YB-1 as necessary for the participation of the protein in the production of virions.
| Original language | English |
|---|---|
| Pages (from-to) | 290-295 |
| Number of pages | 6 |
| Journal | BMB Reports |
| Volume | 51 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2018 |
Bibliographical note
Funding Information:This work was supported by a research grant (2016R1D1A1A02937509 and 2017R1A5A1015366) from the National Research Foundation of Korean Ministry of Science and Technology, and by a grant (HI16C2095) from the Korea Health Industry Development Institute funded by the Ministry of Health and Welfare
Publisher Copyright:
© 2018 by the The Korean Society for Biochemistry and Molecular Biology.
Keywords
- HIV-1
- HIV-1 LTR
- HIV-1 promoter
- Transcription activation
- YB-1
