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Investigation of function and regulation of the YB-1 cellular factor in HIV replication

  • Yu Mi Jung
  • , Kyung Lee Yu
  • , Seong Hyun Park
  • , Seong Deok Lee
  • , Min Jeong Kim
  • , Ji Chang You
  • The Catholic University of Korea
  • Avixgen Inc.

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Y-box binding protein 1 (YB-1) is a member of the cold-shock domain (CSD) protein superfamily. It participates in a wide variety of cellular events, including transcription, RNA splicing, translation, DNA repair, drug resistance, and stress responses. We investigated putative functions of YB-1 in HIV-1 replication. Functional studies using overexpression or knockdown of YB-1 in conjunction with transfection of proviral DNA showed that YB-1 enhances virus production. We found YB-1 regulates HIV-1 production by stimulating viral transcription using HIV-1 LTR sequence U3RU5 with Luciferase assay. We also identified a specific region from amino acids 1 to 324 of YB-1 as necessary for the participation of the protein in the production of virions.

Original languageEnglish
Pages (from-to)290-295
Number of pages6
JournalBMB Reports
Volume51
Issue number6
DOIs
StatePublished - 2018

Bibliographical note

Publisher Copyright:
© 2018 by the The Korean Society for Biochemistry and Molecular Biology.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • HIV-1
  • HIV-1 LTR
  • HIV-1 promoter
  • Transcription activation
  • YB-1

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